By accurately measuring the difference infrared spectrum of Cot-equilibrated bovine carbonic anhydrase against the ethoxzolamideor azide-inhibited enzyme, we have been able to obtain the absorption maximum at 2341 cm-l that is due to the asymmetric stretching of the CO2 molecule loosely bound to a hydrophobic surface at the active site of the enzyme. COz dissolved in water absorbs at 2343.5 cm-...

Pathological rates of gallbladder salt and water transport may promote the formation of cholesterol gallstones. Because prairie dogs are widely used as a model of this event, we characterized gallbladder ion transport in animals fed control chow by using electrophysiology, ion substitution, pharmacology, isotopic fluxes, impedance analysis, and molecular biology. In contrast to the electroneutr...

A general formalism for calculating parameters describing physiological acid-base balance in single compartments is extended to multicompartment systems and demonstrated for the multicompartment example of human whole blood. Expressions for total titratable base, strong ion difference, change in total titratable base, change in strong ion difference, and change in Van Slyke standard bicarbonate...

1. Previous studies of the ventilatory response to metabolic acidosis have usually considered only patients with arterial blood pH above 7-10. To define the response during more severe acidaemia, arterial CO, tension and pH were measured in fifty-three episodes of diabetic ketoacidosis, including twenty-four with pH below 7-10, and ten with pH below 7.00. 2. The relation between arterial CO, te...

Certain bioflavonoids inhibit the glycolysis of variety of tumor cells by interfering with the generation of adenosine diphosphate and inorganic phosphate which are required for glycolysis. Tetra- and pentahydroxy flavones with hydroxyl groups as 3, 3', 4', 5, and 7 (e.g., quercetin) are the most active. They inhibit the activity of isolated Na+-K+-adenosinetriphosphatase of the plasma membrane...

PURPOSE Mutations in SLC4A11, a member of the SLC4 superfamily of bicarbonate transporters, give rise to corneal endothelial cell dystrophies. SLC4A11 is a putative Na⁺ borate and Na⁺:OH⁻ transporter. Therefore we ask whether SLC4A11 in corneal endothelium transports borate (B[OH]₄⁻), bicarbonate (HCO3⁻), or hydroxyl (OH⁻) anions coupled to Na⁺. METHODS SLC4A11 expression in cultured primary ...

This study aimed to evaluate the protective potential of aqueous seed extract ripe Carica papaya fruits on cyanide induced kidney damage in male Wister rats using 24 divided into five groups. The animals were sacrificed at end experimental period and serum harvested for biochemical histological analysis following standard procedures. Estimation function markers such as creatinine urea assessmen...

It has generally been thought that homeostatic mechanisms of renal origin are responsible for minimizing the alkalemia produced by chronic hypocapnia. Recent observations from this laboratory have demonstrated, however, that the decrement in [HCO(-) (3)], which "protects" extracellular pH in normal dogs, is simply the by-product of a nonspecific effect of Paco(2) on renal hydrogen ion secretion...