The lectin hepatic binding protein has a specific binding capacity for desialylated serum glycoproteins and is limited to hepatocyte membranes. This binding capacity was reduced by approximately 60% in the neoplastic nodules which resulted from exposure of rat livers to W-2-acetylaminofluorene. The binding capacity of the primary hepatocellular carcinomas which resulted from this regimen was re...

Glycans or carbohydrates attached to therapeutic glycoproteins can directly affect product quality, safety and efficacy, and therefore must be adequately analyzed and controlled throughout product life cycles. However, the complexity of protein glycosylation poses a daunting analytical challenge. In this study, we evaluated the utility of a lectin microarray for assessing protein glycans. Using...

Analysis of a nuclear family with three affected offspring identified an autosomal-recessive form of spondyloepimetaphyseal dysplasia characterized by severe short stature and a unique constellation of radiographic findings. Homozygosity for a haplotype that was identical by descent between two of the affected individuals identified a locus for the disease gene within a 17.4 Mb interval on chro...

BACKGROUND Complement consists of a complex cascade of proteins involved in innate and adaptive immunity. The cascade can be activated through 3 distinct mechanisms, designated the classical, alternative, and lectin pathways. Although complement is widely accepted as participating in the pathophysiology of ischemia-reperfusion injury, the specific role of the lectin pathway has not been address...

Helicobacter pylori is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. As determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including Helicobacter canis, Helic...

Greg Parsonage, Emily Trebilcock, Marta A Toscano, Germán A Bianco, Juan M Ilarregui, Christopher D Buckley & Gabriel A Rabinovich† Hospital de Clínicas “José de San Martín”, Rabinovich, División Inmunogenética, Facultad de Medicina, Universidad de Buenos Aires, Av. Córdoba 2351, 3 Piso (C1120), Ciudad de Buenos Aires, Argentina Tel.: +54 115 950 8755; Fax: +54 115 950 8758; [email protected]

The lectin hepatic binding protein has a specific binding capacity for desialylated serum glycoproteins and is limited to hepatocyte membranes. This binding capacity was reduced by approximately 60% in the neoplastic nodules which resulted from exposure of rat livers to W-2-acetylaminofluorene. The binding capacity of the primary hepatocellular carcinomas which resulted from this regimen was re...