We present an on-chip microfluidic sample concentrator and detection triggering system for microparticles based on a combination of insulator-based dielectrophoresis (iDEP) and electrical impedance measurement. This platform operates by first using iDEP to selectively concentrate microparticles of interest based on their electrical and physiological characteristics in a primary fluidic channel;...

This paper reports the preparation of cobalt phthalocyanine/layered double hydroxide ultrathin films (UTFs) through an electrostatic layer-by-layer (LBL) technique as well as its application in electrocatalysis for dopamine oxidation. UV-vis absorption and electrochemical impedance spectra indicate the uniform deposition of the LBL films. XRD measurements demonstrate the long-range ordered stru...

Enhancement of biochips and biochips scanners is a crucial issue in the actual industry that needs more sensitive and less expensive molecular analysis tools. In this aim, several points have to be re-examined: detector sensitivity, chip density, labeling. In this perspective, we have developed several different techniques based on soft lithography to pattern molecules. We also have developed a...

A reliable, accurate and rapid multigene-based assay combining real time quantitative PCR (qPCR) and a Razor Ex BioDetection System (Razor Ex) was validated for detection of Xylella fastidiosa subsp. pauca (Xfp, a xylem-limited bacterium that causes citrus variegated chlorosis [CVC]). CVC, which is exotic to the United States, has spread through South and Central America and could significantly...

Electronic interactions at the nanoscale represent one of the fundamental problems of nanotechnology. Excitons and plasmons are the two most typical excited states of nanostructures, which have been shown to produce coupled electronic systems. Here, we explore these interactions for the case of nanowires with mobile excitons and nanoparticles with localized plasmons and describe the theoretical...

We present in this chapter some key technologies for lab-on-a-chip (LOC) applications, in particular related to magnetic transport and optical detection. Our objective is to show how these technologies can be realized and operate synergistically to support integrated biodetection. Magnetic microparticles (“beads”) have proven to be versatile and reliable objects in biomedical analysis and are s...

There is an increasing need to develop novel bioassay methods for low-cost, rapid, and easy-to-use multiplex detection of pathogens in various fields ranging from human infectious disease diagnosis, drinking water quality control, to food safety applications. Due to their unique advantages, magnetic and optomagnetic bioassay principles are particularly promising for biodetection platforms that ...

A recently introduced technique for pathogen recognition called BARDOT (BActeria Rapid Detection using Optical scattering Technology) belongs to the broad class of optical sensors and relies on forward-scatter phenotyping (FSP). The specificity of FSP derives from the morphological information that bacterial material encodes on a coherent optical wavefront passing through the colony. The system...

There is little published data on the performance of hand-portable polymerase chain reaction (PCR) systems that can be used by first responders to determine if a suspicious powder contains a potential biothreat agent. We evaluated 5 commercially available hand-portable PCR instruments for detection of Bacillus anthracis. We used a cost-effective, statistically based test plan to evaluate system...

The rapid and sensitive determination of pathogenic bacteria is extremely important in biotechnology, medical diagnosis, and the current fight against bioterrorism. Current methods either lack ultrasensitivity or take a long time for analysis. Here, we report a bioconjugated nanoparticle-based bioassay for in situ pathogen quantification down to single bacterium within 20 min. The bioconjugated...