This essay examines the historical significance of two APS classic papers that are freely available online: De Léan A, Munson PJ, and Rodbard D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am J Physiol Endocrinol Metab Gastrointest Physiol 235: E97–E102, 1978 (http://ajplegacy.physiology.org/cgi/repri...

A fast and selective HPLC method for assaying amphotericin B in biological samples was developed and validated. The chromatographic separation was achieved in less than 12 min on a reverse-phase C(18) column using an acetonitrile-acetic acid-water (52:4.3:43.7, v/v/v) mixture as mobile phase. The flow rate was 1 mL/min and the effluent was monitored at 406 nm. A linear response over the concent...