We have cloned a genomic DNA sequence encoding the acid protease (PEPA) from Aspergillus oryzae using a 0.6-kb fragment as a probe. This fragment was amplified by the polymerase chain reaction (PCR) using oligonucleotide primers designed from the partial amino acid sequences of peptide fragments of the purified protein. Nucleotide sequencing analysis has shown that the cloned gene (designated p...

The phytopathogenic prokaryote Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BB) of rice and utilizes a type III secretion system (T3SS) to deliver T3SS effectors into rice cells. In this report, we show that the ketoglutarate transport protein (KgtP) is secreted in an HpaB-independent manner through the T3SS of X. oryzae pv. oryzae PXO99(A) and localizes to the ho...

Innate immunity to microorganisms relies on the specific sensing of pathogen-associated molecules by host recognition receptors. Whereas studies in animals have largely focused on the recognition of extracellular pathogen-associated molecules by the TLR (toll-like receptor) superfamily, few studies have been carried out in plants, and it is not understood how these molecules are secreted or mod...

BACKGROUND The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. RESULTS Before lignocellulose hydrolysate fractions were tested as substrates, experiments wit...

Kumar U, Joshi AK, Kumar S, Chand R and Röder MS Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005 India; Department of Biotechnology, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut 250110, India; Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu Univers...

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae gl...

flour beetles, Tribolium confusum. Efficacy was assessed on wheat and maize at three dosages of spinosad dust formulation (corresponding to 0.0625, 0.1875 and 0.625ppm of active ingredient [AI] for S. oryzae and to 0.1875, 0.625 and 1.25 ppm of AI for T. confusum), alone or combined with SilicoSec at 150ppm for S. oryzae and 250 ppm for T. confusum. The mortality of S. oryzae exposed for 14d on...

Kosakonia oryzae KO348 is an endophytic and plant growth-promoting strain isolated from the roots of rice in Italy. Here, we report the draft genome sequence of Kosakonia oryzae KO348.

The present study investigates the effects of varying levels of dietary fermented Aspergillus oryzae (0 g Kg-1 as control group, 10, 20 and 30 g Kg-1) on performance and haemato-immunological indices of common carp (Cyprinus carpio) fingerlings. Common carp fingerlings (n=240, w=4.56±0.17 g) were supplied, randomly stocked in 12 aquaria and fed with experimental diets for 7 weeks. The results r...