The biuret method offers some advantages as a simple, rapid photometric method for the determination of proteins. In the methods which have been proposed, precipitation of cupric hydroxide gives rise to certain difficulties (1, 2). It occurred to us that the precipitates might be avoided by the addition of a reagent which would form a compound with copper which was soluble in alkali, but of suc...

There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in the assay of biopharmaceuticals. The techniq...

BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...

There are the dye-binding method, the biuret method, and the turbidimetric method for determining urine protein. Of the turbidimetric methods, a SSA method that is highly sensitive and reliable is used not only to determine the urine protein concentration but also to confirm a false-positive in a test strip and to screen the Bence-Jones proteins from the discrepancy between the test strip and S...

Rhodococcus sp. strain Mel was isolated from soil by enrichment and grew in minimal medium with melamine as the sole N source with a doubling time of 3.5 h. Stoichiometry studies showed that all six nitrogen atoms of melamine were assimilated. The genome was sequenced by Roche 454 pyrosequencing to 13× coverage, and a 22.3-kb DNA region was found to contain a homolog to the melamine deaminase g...