Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible for the reduced catalytic efficiency against certain cephalosporins, including ceftazidime and cefepime.

Objective: The aim of this study was to determine the effects of residues at 228th positions of VIM-38 on minimum inhibition concentration (MIC) and molecular docking analysis. Materials and methods: blaVIM-38 gene was cloned into expression vector pET100/D-TOPO. Then pET100/D-TOPO-VIM-38 was used to generate the R228S mutation by site-directed mutagenesis. The mutant was transformed into E. co...

Metallo-β-lactamases (MBLs) are of increasing clinical significance; the development of clinically useful MBL inhibitors is challenged by the rapid evolution of variant MBLs. The Verona integron-borne metallo-β-lactamase (VIM) enzymes are among the most widely distributed MBLs, with >40 VIM variants having been reported. We report on the crystallographic analysis of VIM-5 and comparison of bioc...

INTRODUCTION Medically refractory tremor treatment has evolved over the past half-century from intraoperative thalamotomy to deep brain stimulation (DBS) of the thalamic ventral intermediate nucleus (VIM). Within the past 15years, unilateral radiosurgical VIM thalamotomy has emerged as a comparably efficacious treatment modality. METHODS An extensive literature search of VIM DBS series was pe...

Identification of Pseudomonas aeruginosa Clinical Strains Harboring the blaVIM-2 Metallo-β-Lactamase Gene in Akita Prefecture, Japan Jun Yatsuyanagi*, Shioko Saito, Yuko Ito, Kazuko Ohta, Jun Kato, Seizaburo Harata, Noriyuki Suzuki and Ken-ichi Amano Akita Prefectural Institute of Public Health, Akita 010-0874, Akita Kumiai General Hospital, Akita 011-0911, Odate Municipal Hospital, Oodate 027-...

Abstract Background Diarrhoea is a major cause of childhood disease in the developing countries. This experimental study investigated prevalence ESBL and MBL genes enteropathogenic strains Escherichia coli Salmonella spp. isolated from diarrheagenic children Awka, Nigeria. Methods Two hundred stool samples were collected diarrhea patients three paediatric hospitals within Awka metropolis, All E...

Abstract Background The irrational use of carbapenems in the last years lead to emergence carbapenem-resistant Enterobacteriaceae (CRE). This study aimed at determining prevalence CRE intestinal carriage among admitted patients a tertiary care hospital Egypt, characterize carbapenemase-producing genes and identify possible risk factors colonization. One hundred rectal swabs were collected from ...