Chitosan has been broadly utilized in bone scaffold production because of its antibacterial qualities, low toxicity, biodegradability, biocompatibility, and ability to aid regeneration processes wound healing. In this work, chitosan was produced from crab shell waste through demineralization, deproteination, deacetylation, utilizing HCl 1:7 (v/v), NaOH 3%, 1:10 (v/w), 50%. The aim study is exam...

Cerny H.: Structural Evaluation of the Cartilage and Bone Tissue in Cattle from the Functional and Morphological Aspects. Acta vet. Brno, 49, 1980: 127-144. The evaluation of morphological structures with regard to changes induced by disorders of mineral metabolism in 8 to 12-month old bulls was performed in 82 cylinders of bone tissue obtained by osteobiopsy of the tuber coxae using a modified...

Endochondral bone formation was induced in postnatal rats by implantation of demineralized rat bone matrix. Corresponding control tissue was generated by implanting inactive extracted bone matrix, which did not induce bone formation. At various times, implants were removed and sequentially extracted with guanidine hydrochloride, and then EDTA and guanidine hydrochloride. Transforming growth fac...

OBJECTIVE The aim of this study was to investigate the correlation between the demineralization depth measured by dental computed tomography (CT) and the measured value from a non-destructive dental caries diagnosis with a laser. METHODS Optimal cut-off points were obtained for enamel and dentin caries, from the measured levels from a dental caries diagnosis with a laser, to investigate the c...

This study aimed to examine the anti-demineralization capacities of (a) tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) and 950 ppm fluoride paste, (b) casein phosphopeptide amorphous calcium phosphate paste and (c) 950 ppm fluoride solution using optical coherence tomography (OCT). Enamel blocks were cut from the bovine incisors and treated using one of the above-mention...

The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37 degrees C, 10% CO2. To vali...

The application of high-resolution analytical techniques to characterize ancient bone proteins requires clean, efficient extraction to obtain high quality data. Here, we evaluated many different protocols from the literature on ostrich cortical bone and moa cortical bone to evaluate their yield and relative purity using the identification of antibody-antigen complexes on enzyme-linked immunosor...

The report of the World Health Organization (WHO) of 2008 defines osteoporosis as a disease characterized by low bone mass and an increased risk of fracture. Postmenopausal osteoporosis is connected to the decrease in estrogens concentration as a result of malfunction of endocrine ovarian function. Low estrogens concentration causes increase in bone demineralization and results in osteoporosis....

In the United States, demineralized bone matrix (DBM) is considered a transplantable tissue and therefore is regulated primarily by the American Association of Tissue Banks. Even though DBM is not subjected to the same regulations relative to performance claims as medical devices are, one would expect different processing methods might yield DBM preparations of different osteoinductive potentia...