Reference antisera were produced against 15 influenza hemagglutinin (HA) subtypes using DNA vaccination to produce a high-quality polyclonal serum to the HA protein without antibodies to other influenza viral proteins. The HA gene from each of 15 different HA subtypes of influenza virus was cloned into a eukaryotic expression vector and injected intramuscularly, together with a cationic lipid, ...

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a global threat. The only approved vaccine against TB, Mycobacterium bovis bacillus Calmette-Guérin (BCG), provides insufficient protection and, being a live vaccine, can cause disseminated disease in immunocompromised individuals. Previously, we found that intradermal cDNA tattoo immunization with cDNA of tetanus toxoid fra...

this paper presents the new topology of three phase on line uninterruptible power supply (ups) by employing t-source inverter (tsi) with maximum constant boost pulse width modulation (pwm) control and the results are compared with the conventional uninterruptible power supply (ups). the conventional ups consists of voltage source inverter (vsi) with step up transformer or dc-dc booster which de...

BACKGROUND The development of a successful new tuberculosis (TB) vaccine would circumvent many limitations of current diagnostic and treatment practices. However, vaccine development is complex and costly. We aimed to assess the potential cost effectiveness of novel vaccines for TB control in a sub-Saharan African country--Zambia--relative to the existing strategy of directly observed treatment...

In situ vaccination is a promising immunotherapeutic approach, where various local ablative therapies are used to induce an immune response against tumor antigens that released from the therapy-killed cells. We recently proposed using intratumoral gene electrotransfer for concomitant transfection of cytotoxic cytokine necrosis factor-? (TNF?) in vaccination, and immunostimulatory interleukin 12...