introduction: the value of embryonic stem-like cells in cloning is obvious. production of cloned animals can be achieved by introduction of these cells into a tetraploid embryo. tetraploid embryo is used as a feeder for development of embryonic stem-like cells and can be produced in vitro by electro-fusion of 2-cell embryos. the aim of this study was to ass,essthe effect of voltage alteration a...

BACKGROUND Progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period. OBJECTIVE The objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development. MATE...

Primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (Vero) for the isolation and seroneutralization of rinderpest virus.

Abstract Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning cattle and buffaloes, generating economic losses related the death due heart failure, miscarriage, abortion, stillbirth, neonatal mortality. The aim this study was examine embryotoxic potential aqueous extract on vitro bovine embryos. In performed five replicates embr...

PURPOSE To study in both in situ and primary cultures the posttranslational phosphorylation of connexin46 (Cx46), one of two members of the connexin family of gap junction proteins expressed by lens fibers. METHODS Phosphatase digestion, gel electrophoresis, cell culture, organ culture, immunoprecipitation, metabolic labeling, and phosphoamino acid analysis were the methods used in this study...

Altered patterns of gene expression and the imprinted status of genes have a profound effect on cell physiology and can markedly alter embryonic and fetal development. Failure to maintain correct imprinting patterns can lead to abnormal growth and behavioural problems, or to early pregnancy loss. Recently, it has been reported that the Igf2R and Grb10 genes are biallelically expressed in sheep ...

Abstract Background The developmental competence of oocyte derived from in vitro maturation (IVM) is significantly lower than those matured vivo. Rapamycin an immunosuppressive substance and widely used to prolong the survival preserve cells culture. objective this research was explore whether rapamycin supplementation during IVM enhances meiotic maturation, quality, subsequent embryonic develo...

Objective(s): Here, we examined the function of our produced monoclonal antibody (mAb10C3) to recognize one of the most important members of the HEAT shock factor family, Hsf5, in embryonic development and in spermatogenic cells of adult mouse testis.Materials and Methods: The targeting effects of mAb10C3 were investigated by immunohisto...

Extracellular vesicles (EVs) have been identified within different body fluids and cell culture media. However, there is very little information on the secretion of these vesicles during early embryonic development. The aims of this work were first to demonstrate the secretion of extracellular vesicles by pre-implantation bovine embryos and second to identify and characterize the population of ...