Betulinic acid (BA) is a pentacyclic triterpenoid acid obtained from the stem bark of Tecomella undulata Seem. (Bignoniaceae). Development of an efficient extraction method for the isolation of BA is important as it has a wide range of pharmacological activity. A Box-Behnken design (BBD) was used to investigate the effect of extraction variables such as temperature (30-60 °C), time (4-8 h) and ...

The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities. The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation tempe...

A Box-Behnken design (BBD) was applied to optimize the ultrasound-assisted extraction of polysaccharides from mulberry leaves. Under the optimum conditions of an extraction temperature of 57 °C, an extraction time of 80 min and a liquid/solid ratio of 53 mL/g, the mulberry leaf polysaccharide (MLP) yield was 6.92 ± 0.29%. Then, three fractions of MLPs were obtained by deproteinization, dialysis...

In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (r...

In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from Brosimum alicastrum leaf and to study the radical-scavenging activity, a three-level three-factor Box-Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature ...

BACKGROUND Discharge of grey wastewater into the ecological system causes the negative impact effect on receiving water bodies. METHODS In this present study, electrocoagulation process (EC) was investigated to treat grey wastewater under different operating conditions such as initial pH (4-8), current density (10-30 mA/cm2), electrode distance (4-6 cm) and electrolysis time (5-25 min) by usi...

Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) are the mostcommon contaminants in cereals worldwide, causing a wide range of adverse health effects onanimals and humans. Many environmental factors can affect the production of these mycotoxins.Here, we have used response surface methodology (RSM) to optimize the Fusarium graminearumstrain 29 culture conditions for maximal toxin p...

The presence of humic acid (HA) in water treatment processes is very harmful and the cause of undesirable color, taste, and smell. Drinking water containing high concentrations of humic substances can be the cause of many health problems. Therefore, the removal of these compounds from water resources is a very important topic. In this research, response surface methodology (RSM) has been used t...

In the present research, salting out and vortex assisted dispersive liquid–liquid microextraction based on solidification of floating organic drop microextraction (SO-VADLLME-SFODME)  for the extraction and determination of phenol and chlorophenols in water samples followed by HPLC have been studied through a novel, simple, low-cost, selectable and environment friendly (so called green) method....

To improve the pod-picking efficiency of combine harvester for both peanut seedlings and peanuts, a longitudinal axial flow device is designed in this study. The fixation adjustment modes rod were determined. roller’s rotational speed, diameter, roller, effective rod-picking length, screw-feeding stirrer’s critical parameters determined by theoretical calculation. A combined design quadratic re...