Sphingolipids are essential structural components of cellular membranes, playing prominent roles in signal transduction that governs cell proliferation, differentiation and apoptosis. Ceramides, a family of distinct molecular species characterized by various acyl chains, are synthesized de novo at the cytosolic side of the endoplasmic reticulum serving as precursors for the biosynthesis of sphi...

We studied the effects of mexidol (3-oxy-6-methyl-2-ethylpiridine succinate) on antioxidant glutathione system in rat brain mitochondria experimental Parkinson’s disease induced by rotenone administration. Wistar rats were divided into following groups 6 each: I - intact (control); II (3 mg/kg per day) was injected subcutaneously for 2 weeks; III after intoxication, (50 intraperitoneally weeks....

Cytochrome oxidase (CO) is a mitochondrial energy-generating enzyme used in brain studies as a marker of neural functional activity. The activity of CO in different brain regions, revealed histochemically, is distributed nonhomogeneously but in distinct patterns. Localized differences in CO activity could arise from localized differences in enzyme amount or from localized regulation of enzyme t...

Mitochondria are dynamic organelles critical for many cellular processes, including energy generation. Thus, mitochondrial dysfunction likely plays a role in the observed alterations in brain glucose metabolism during aging. Despite implications of mitochondrial alterations during brain aging, comprehensive quantitative proteomic studies remain limited. Therefore, to characterize the global age...

Introduction: Chronic ethanol consumption leads to significant functional and structural changes in the mitochondria of heart brain, increasing generation reactive oxygen species. Therefore, search for substances, which improve state and, meantime, reduce oxidative stress, is relevant.
 Materials methods: 10-months-old Wistar female rats were used experiments. alcohol intoxication (CAI) wa...

1. The effects of phenylpyruvate, a metabolite produced in phenylketonuria, on the pyruvate dehydrogenase-complex activity were investigated in rat brain mitochondria. 2. Pyruvate dehydrogenase activity was measured by two methods, one measuring the release of 14CO2 from [1-1"C]pyruvate and the other measuring the acetyl-CoA formed by means of the coupling enzyme, pigeon liver arylamine acetylt...