Exogenous nitrogen (N) inputs greatly change the emission and uptake of carbon dioxide (CO2) methane (CH4) from temperate grassland soils, thereby affecting (C) budget regional terrestrial ecosystems. Relevant research focused on natural grassland, but effects N fertilization C exchange fluxes different forage soils driving mechanisms were poorly understood. Here, a three-year addition experime...

Cells of the terrestrial plant species bromegrass (Bromus inermis L.) are not naturally adapted to withstand the hydrostatic pressures encountered in aquatic environments. However, after treatment with the natural plant growth hormone abscisic acid (75 micromolar), bromegrass cells survived a hydrostatic pressure of 101.3 megapascals, approximating the limits of ocean depth (10,860 m). The incr...

PUF proteins are a conserved family of RNA binding proteins found in all eukaryotes examined so far. This study focussed on PUF5, one of 11 PUF family members encoded in the Trypanosoma brucei genome. Native PUF5 is present at less than 50000 molecules per cell in both bloodstream and procyclic form trypanosomes. C-terminally myc-tagged PUF5 was mainly found in the cytoplasm and could be cross-...

Control of gene expression in trypanosomes relies almost exclusively on post-transcriptional mechanisms. Trypanosomes have the normal enzymes for mRNA decay: both the exosome and a 5'-3'-exoribonuclease are important in the degradation of very unstable transcripts, whereas the CAF1/NOT complex plays a major role in the degradation of all mRNAs tested. Targeted RNA interference screening was use...

Recent climatic trends, such as warming temperatures, decrease in rainfall, and extreme weather events (e.g., heatwaves), are negatively affecting the performance of forests. In northern Patagonia, conditions have caused tree growth reduction, crown dieback, massive die-back events. However, studies looking at these consequences southernmost temperate forest (Nothofagus betuloides Nothofagus pu...