Melioidosis is highly prevalent in Northeast Thailand which is associated with high incidence of Burkholderia pseudomallei present in the soil of this region. B. pseudomallei when present in biofilm becomes resistant to numerous environmental factors and also to certain antibiotics. In this study, we examined the effects of several environmentally relevant factors (salinity, iron, manganese and...

Table S1. Bacterial strains used to examine the host range of bacteriophage E202. Bacterial strain Source or reference Plaque formation Burkholderia mallei NCTC 120 NCTC NCTC 10248 “ + NCTC 10229 “ + NCTC 10260 “ + NCTC 10247 “ + NCTC 3708 “ + NCTC 3709 “ + ATCC 23344 ATCC + ATCC 10399 “ + ATCC 15310 “ + DB110795 [1] DD3008 [2] + Burkholderia pseudomallei 316c [3] NCTC 4845 NCTC + 1026b [4] WR...

Burkholderia pseudomallei, the causative agent of melioidosis was found in a small cluster of cases in Tejuçuoca, Ceará, Brazil. Tests were carried out to determine its phenotypic characteristics: colony morphology on Ashdown agar and MacConkey agar, biochemical profile in conventional biochemical tests and API 20NE, arabinose assimilation and susceptibility testing by disk diffusion, comparing...

Burkholderia mallei lipopolysaccharide (LPS) has been previously shown to cross-react with polyclonal antibodies raised against B. pseudomallei LPS; however, we observed that B. mallei LPS does not react with a monoclonal antibody (Pp-PS-W) specific for B. pseudomallei O polysaccharide (O-PS). In this study, we identified the O-PS biosynthetic gene cluster from B. mallei ATCC 23344 and subseque...

Acute melioidosis may present as localised or septicaemic infections and can be fatal if left untreated. Burkholderia pseudomallei resistant to antibiotics used for the treatment of melioidosis had been reported. The aim of this study was to determine the in vitro antibiotic susceptibility patterns of Burkholderia pseudomallei isolated in Malaysia to a panel of antibiotics used for the treatmen...

Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. Only a few antibiotic selection markers, including gentamicin, kanamycin, and zeocin, are currently approved for use with this bacterium, but wild-type strains are highly resistant to these antibiotics. To facilitate routine genetic manipula...

reference spectra for the organism and its close relatives (e.g., B. thailandensis). B. pseudomallei, although different from other Burkholderia spp. in its pathogenicity and epidemiology, is not easily discriminated from B. thailandensis or B. cepacia complex by using phenotypic tests (10). In summary, infection with B. pseudomallei should be considered in patients with pneumonia after travel ...