BACKGROUND Refactoring microorganisms for efficient production of advanced biofuel such as n-butanol from a mixture of sugars in the cheap feedstock is a prerequisite to achieve economic feasibility in biorefinery. However, production of biofuel from inedible and cheap feedstock is highly challenging due to the slower utilization of biomass-driven sugars, arising from complex assimilation pathw...

The finite nature of fossil-based resources, as well as the environmental awareness of the effects of their exploitation, has led to an increasing interest in finding alternative ways to produce chemicals, materials and energy. In terms of energy, biofuels are a good option for decreasing the environmental effects caused by fossil fuels, and in the last years, a great effort has been made to fi...

five flavonoid glycosides were isolated from the n-butanol soluble fraction of the ethanolic extract of rapistrum rugosum and their structures were assigned from 1h- and 13c-nmr spectra (dept) with 2d nmr as quercetin-3-o-α-l-rhamnopyranoside (1), quercetin-3-o-β-d-xyloside (2), quercetin, 3-o-α-l-arabinopyranoside,7-o-α-l-rhamnopyranoside (3), kaempferol 3-o-α-l-arabinopyranoside, 7-o-α-l-rham...

A clostridial strain has been isolated that produced n-butanol, ethanol, butyrate, and acetate as major fermentation products from glucose but no acetone. At a pH of 6.6, n-butanol was formed by this microorganism only during growth. On the basis of its physiological characteristics and DNA-DNA homology data, the strain was assigned to the "Clostridium tetanomorphum" group (S. Nakamura, I. Okad...

Volatile organic compounds (VOCs), 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol, were detected together with β-cyclocitral from the cyanobacterium Microcystis aeruginosa NIES-843. These alcohols were optimally produced after 35 d of culture, during which nitrate nitrogen in the cultured broth became exhausted. Additionally, these alcohols were definitely produced using the 2-keto-...

Activities of separated and chromatographed substances in the nonacidic, acidic ethyl acetate and acidic butanol fractions from bean seeds, Phaseolus vulgaris L., cv. Bountiful and Kentucky Wonder, were measured in the Progress No. 9 dwarf pea bioassay grown under red light. Activity in the nonacidic fraction was shown to be attributable only to neutral substances and was free of acidic gibbere...

Solvents toxicity is a major limiting factor hampering the cost-effective biotechnological production of chemicals. In Clostridium acetobutylicum, a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was identified. Disruption of SMB_G1518 and/or its downstream gene SMB_G1519 resulted in increased butanol tolerance, while overexpression of SMB_G15...

Three phase partitioning (protein precipitate obtained as an interfacial layer between lower aqueous and upper t-butanol phases, formed by the addition of ammonium sulphate and t-butanol to the aqueous solution of protein) followed by lyophilization in the presence of two-component excipient resulted in 400-480x increases in transesterification activity of lyophilized powders of subtilisin Carl...

Biological and chemocatalytic processes are tailored in order to maximize the production of sustainable biodiesel from lignocellulosic sugar. Thus, the combination of hydrotalcite-supported copper(II) and palladium(0) catalysts with a modification of the fermentation from acetone-butanol-ethanol to isopropanol-butanol-ethanol predictably produces higher concentrations of diesel-range components...