نتایج جستجو برای: c bcl 2 proteins

تعداد نتایج: 3591131  

2011
Christelle Villeneuve Laurent Baricault Ludovic Canelle Nadia Barboule Carine Racca Bernard Monsarrat Thierry Magnaldo Florence Larminat

Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action remains incompletely understood. Deciphering the network of Bcl-2 interacting factors is necessary to better understand the key function of Bcl-2 in apoptosis initiation. To identify novel Bcl-2 mitochondrial partners, we have combined a Bcl-2 immunocapture with a mass spectrometry analysis using high...

Journal: :The Journal of biological chemistry 2002
Shirley Gil-Parrado Amaury Fernández-Montalván Irmgard Assfalg-Machleidt Oliver Popp Felix Bestvater Andreas Holloschi Tobias A Knoch Ennes A Auerswald Katherine Welsh John C Reed Hans Fritz Pablo Fuentes-Prior Eberhard Spiess Guy S Salvesen Werner Machleidt

Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, but the underlying mechanism(s) remain(s) to be elucidated. We examined ionomycin-induced cell death in LCLC 103H cells, derived from a human large cell lung carcinoma. We detected hallmarks of apoptosis such as membrane blebbing, nuclear condensation, DNA ladder formation, caspase activation, and poly-(ADP-ri...

2009
Sanjeev Gupta George EN Kass Eva Szegezdi Bertrand Joseph

The mitochondrial pathway to apoptosis is a major pathway of physiological cell death in vertebrates. The mitochondrial cell death pathway commences when apoptogenic molecules present between the outer and inner mitochondrial membranes are released into the cytosol by mitochondrial outer membrane permeabilization (MOMP). BCL-2 family members are the sentinels of MOMP in the mitochondrial apopto...

ژورنال: :مجله دانشگاه علوم پزشکی زنجان 0
ایرج جعفری انارکولی i jafari anarkooli مجتبی سنکیان m sankian شهریار احمدپور sh ahmadpour حسین حقیر h haghir شکوفه بنکداران sh bonakdaran عیدالرضا وارسته a varasteh

چکیده زمینه و هدف: دیابت قندی یک اختلال مزمن متابولیک همراه با افزایش قند خون می باشد که به طور نامطلوبی هر دو سیستم عصبی مرکزی و محیطی را درگیر می کند. از آن جا که ژن های خانواده ی bcl-2 نقش مهمی در تنظیم آپوپتوز دارند، هدف از مطالعه ی حاضر بررسی اثرات انسولین و اسیداسکوربیک بر بیان ژن های خانواده ی bcl-2 نظیر bax (القا کننده ی آپوپتوز)، bcl-2 و bcl-xl (مهارکننده ی آپوپتوز) در هیپوکامپ موش های...

Journal: :Metabolic engineering 2014
Neil Templeton Abasha Lewis Haimanti Dorai Elaine A Qian Marguerite P Campbell Kevin D Smith Steven E Lang Michael J Betenbaugh Jamey D Young

Anti-apoptosis engineering is an established technique to prolong the viability of mammalian cell cultures used for industrial production of recombinant proteins. However, the effect of overexpressing anti-apoptotic proteins on central carbon metabolism has not been systematically studied. We transfected CHO-S cells to express Bcl-2∆, an engineered anti-apoptotic gene, and selected clones that ...

2016
Laurel T. Bate-Eya Ilona J.M. den Hartog Ida van der Ploeg Linda Schild Jan Koster Evan E. Santo Ellen M. Westerhout Rogier Versteeg Huib N. Caron Jan J. Molenaar M. Emmy M. Dolman

The anti-apoptotic protein B cell lymphoma/leukaemia 2 (BCL-2) is highly expressed in neuroblastoma and plays an important role in oncogenesis. In this study, the selective BCL-2 inhibitor ABT199 was tested in a panel of neuroblastoma cell lines with diverse expression levels of BCL-2 and other BCL-2 family proteins. ABT199 caused apoptosis more potently in neuroblastoma cell lines expressing h...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
Jerry E Chipuk John C Fisher Christopher P Dillon Richard W Kriwacki Tomomi Kuwana Douglas R Green

Normal cellular lifespan is contingent upon preserving outer mitochondrial membrane (OMM) integrity, as permeabilization promotes apoptosis. BCL-2 family proteins control mitochondrial outer membrane permeabilization (MOMP) by regulating the activation of the pro-apoptotic BCL-2 effector molecules, BAX and BAK. Sustainable cellular stress induces proteins (e.g., BID, BIM, and cytosolic p53) cap...

Journal: :The Biochemical journal 2012
Elena S Dremina Victor S Sharov Christian Schöneich

We have demonstrated previously that Bcl-2 and Bcl-2Δ21, a C-terminally truncated Bcl-2 sequence, inactivate SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) 1 in isolated SR (sarcoplasmic reticulum), accompanied by a translocation from CRDs (caveolae-related domains) of the SR. In the present study, we obtained evidence for the interaction of Bcl-2 with SERCA2b in C2C12 myoblasts and H...

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