The interaction of native calf thymus DNA (ctDNA) with solifenacin succinate (SFS) was investigated under simulated physiological conditions by multi-spectroscopic techniques and viscometric measurements. It concluded that SFS could intercalate into the base pairs of ctDNA, and the fluorescence quenching by ctDNA was static quenching type. Thermodynamic parameters calculated suggested that the ...

Nuclei of calf thymus and liver and of rat liver were isolated in sucrose media and a number of their properties studied in relation to those of corresponding nuclei isolated in non-aqueous media with a view to determining their capacity to retain soluble components. The best preparations of sucrose nuclei were obtained from calf thymus. Cytochrome oxidase measurements and DNA/N ratios were far...

Using absorption measurements the reassociation kinetics of three satellite DNA components isolated from calf thymus was studied under various conditions. A different method using CsC1 density gradient determinations particularly suited for kinetic analysis of mixtures was also used and shown to give similar results. Reassociation rate constants were corrected for mismatching during strand reas...

Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence of a 5' to 3' exonuclease activity that is specific for a single strand of duplex DNA. This activity is capable of degrading a 3'-labeled oligonucleotide hybridized to M13mp18 DNA. When a second oligonucleotide primer is annealed 3 bases upstream, degradation of the downstream primer is s...

background: development of safe radioprotector is a challenging task. in this study radioprotective effect of sucrose has been demonstrated in calf thymus dna (ctdna). sucrose is a free radical scavenger and also acts as osmolyte and therefore can influence the water activity around dna and effects of radiation on dna. hoechst 33258 was used to probe the possible alteration in physicochemical p...

A procedure is described for obtaining DNA-dependent RNA polymerase form B from calf thymus in high purity. The procedure includes homogenization in low salt, sedimentation of chromatin at 50 000 x g, adsorption to DEAE-cellulose in a batch procedure, DNA-agarose chromato­ graphy, and chromatography on DEAE-Sephadex. Analyses of the preparation by band sedimenta­ tion and gel-electrophoresis in...

The interaction of three second-generation anthracycline deriv atives with polynucleotides, supercoiled DNA, and calf thymus nucleosomes has been studied by terbium fluorescence mea surements and agarose gel electrophoresis. It was shown that, as expected, A/-trifluoroacetyladriamycin-14-valerate had little detectable effect on the fluorescence of this guanine-specific probe except for polydisp...

The enzymatic DNA relaxation requires the DNA to be transiently nicked and rejoined, the covalent topoisomerase-DNA complex being a key intermediate of the nicking-joining reaction. Practically, this reaction is most often characterized by oligonucleotides. However, the incision-religation of an oligonucleotide does not fully recapitulate the incision-religation occuring during relaxation and t...

New symmetrical acyclic binuclear copper (II) complexes [CuL](ClO4)2 1 and 2 were synthesized by template method, from bicompartmental ligand 2,6-bis((E)-(2-(2aminoethylamino)ethylimino)methyl)-4-methylphenol (L) and 2-((7E)-(2-((Z)-2-((naphthalen-1-yl) methyleneamino)ethylamino)ethylimino)methyl)-6-((9E)-(2-((Z)-2-((naphthalen-1-yl)methyleneamino) ethylamino)ethylimino) methyl)-4-methylphenol ...

Abstract Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because its stable thermodynamic, kinetic and redox properties. Novel hexaferrocenium tri[hexa(isothiocyanato)iron(III)]trihydroxonium (HexaFc) complex was product from reaction ferrocene, maleic acid ammonium thiocyanate confirmed by elemental analysis CHNS, FTIR single crystal X-ray crystallogra...