We have used calretinin antibodies to label selectively the mosaic of AII amacrine cells in the macaque retina. Confocal analysis of double-labeled material indicated that AII dendrites spiral down around descending rod bipolar axons before enveloping the synaptic terminals. Processes from a previously observed dopaminergic plexus in the inner nuclear layer were observed to contact the somata o...

IHC é uma disciplina jovem e há relativamente poucas teorias próprias e de amplo espectro propostas até hoje. A Engenharia Semiótica é uma destas teorias. Organizada de maneira completa há apenas poucos anos, ela foi inicialmente ilustrada com casos da prática profissional de IHC. Este tutorial apresenta seus aportes para a prática científica de IHC, no âmbito da Ciência da Computação. Palavras...

We assessed the validity of immunoblotting, immunohistochemistry (IHC), and immunocytochemistry (ICC) to detect anti-neuronal antibodies in an attempt to establish a diagnostic approach for pediatric autoimmune encephalitis. Both IHC and ICC had higher sensitivity than immunoblotting and could differentiate between antibodies directed towards intracellular and cell surface antigens. There was a...

This study sought to evaluate a new combined gene and protein detection platform in the context of HER2 evaluation in breast and gastric carcinomas. HER2 immunohistochemistry (IHC) and dual color in situ hybridization (Dual ISH) were combined on a single slide. Results were compared with conventional HER2 IHC and fluorescence ISH. Results from the gene and protein assay were reliable and highly...

some physicochemical parameters of honey have been introduced by the international honey commission to evaluate its quality and origin but processes such as heating and filtering can affect these parameters. in traditional iranian medicine, deslagging process involves boiling honey in an equal volume of water and removing the slag formed during process. the aim of this study was to determine th...

Molecular classification of diseases based on multigene expression signatures is increasingly used for diagnosis, prognosis, and prediction of response to therapy. Immunohistochemistry (IHC) is an optimal method for validating expression signatures obtained using high-throughput genomics techniques since IHC allows a pathologist to examine gene expression at the protein level within the context...

This study aimed to re-evaluate the concordance between FISH and IHC for HER-2 status, and evaluate its clinicopathologic significance in breast cancer by the new guideline (2014) in China. We determined the HER-2 status in 589 cases of invasive breast cancer, the concordance and correlation between these two results and their relationship to cliniopathological characteristics were evaluated. T...

Background. The feasibility and accuracy of immunohistochemistry (IHC) on frozen sections, when assessing sentinel node (SN) status intraoperatively in breast cancer, is a matter of continuing discussion. In this study, we compared a center using IHC on frozen section with a center not using this method with focus on intraoperative diagnostic values. Material and Methods. Results from 336 patie...

To test the feasibility of semiquantitative immunohistochemical staining (IHC) with large sized gold conjugated secondary antibody (gold-2 degrees Ab), we used beads covered with a known amount of primary antibody and a secondary antibody conjugated with gold colloid particles (20 and 40 nm in diameter), and we compared the results to those obtained by enzyme IHC. Beads coated with 6 graded amo...