نتایج جستجو برای: camv 35s promoter
تعداد نتایج: 92532 فیلتر نتایج به سال:
Miraculin là một protein tự nhiên có khả năng chuyển vị chua thành ngọt mặc dù đặc tính của nó không ngọt. Trong nghiên cứu này, mã di truyền miraculin được tối ưu. Ngoài ra, nhằm tăng cường sự biểu hiện trong cây cà gen, promoter E8 hiệu ở quả từ và terminator HSP A.thaliana cũng sử dụng để điều khiển hoạt động gen tổng hợp. Kết phân tích bằng PCR cho thấy 16 dòng mang cấu trúc E8-pro:Mir:HSP-...
High stability of transgene expression is essential for functional genomics studies using transformation approaches and for application of genetic engineering to commercial forestry. We quantified expression of two reporter genes, green fluorescent protein (GFP) and the herbicide bialaphos resistance gene (BAR), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitr...
Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different p...
Fragaria vesca was transformed with a transposon tagging construct harbouring amino terminally deleted maize transposase and EGFP (Ac element), NPTII, CaMV 35S promoter (P35S) driving transposase and mannopine synthase promoter (Pmas) driving EGFP (Ds element). Of 180 primary transgenics, 48 were potential launch pads, 72 were multiple insertions or chimaeras, and 60 exhibited somatic transposi...
Most of the plant homeodomain-containing proteins play important roles in organ patterning and development, and Arabidopsis GLABRA2 (GL2), a member of the class IV homeodomain-leucine zipper (HD-ZIP) proteins, is a trichome and non-root hair cell regulator. Here we report the analysis of two cotton homeodomain-containing proteins, GaHOX1 and GaHOX2, isolated from the diploid cotton Gossypium ar...
Epigenetic modification generally refers to phenotypic changes by a mechanism other than changes in DNA sequence and plays a significant role in developmental processes. In this study, we found that overexpression of one alternatively spliced tomato DDB1 transcript, DDB1(F) that is prevalently present in all tested tissues, resulted in reduction of organ size. Transgenic plants constitutively e...
The full-length cDNA of a Taiwan strain of Zucchini yellow mosaic virus (ZYMV TW-TN3) was constructed from five overlapping cDNA clones downstream from the bacteriophage T7 promoter in plasmid pT7ZYMV25. The plasmid was able to generate an in vitro transcript corresponding to TW-TN3 (9591 nt) with one extra guanosine residue at the 5 ́ terminus and a poly(A)95 tract at the 3 ́ end. In addition, p...
specific hybridization has reduced disease problems, increased biomass yield and sugar yield, and improved Direct screening of genomic libraries for highly expressed genes adaptability for growth under various stress conditions is an efficient way to identify promoters that confer high levels of (Roach, 1972; Srivastava et al., 1994). gene expression. To test the efficacy of this approach to is...
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