Diamond Blackfan Anemia (DBA) is an inherited bone marrow failure syndrome with clinical features of red cell aplasia and variable developmental abnormalities. Most affected patients have heterozygous loss of function mutations in ribosomal protein genes but the pathogenic mechanism is still unknown. We generated induced pluripotent stem cells from DBA patients carrying RPS19 or RPL5 mutations....

CD147 null mutant male mice are infertile with arrested spermatogenesis and increased apoptotic germ cells. Our previous studies have shown that CD147 prevents apoptosis in mouse spermatocytes but not spermatogonia. However, the underlying mechanism remains elusive. In the present study, we aim to determine the CD147-regulated apoptotic pathway in mouse spermatocytes. Our results showed that im...

A key aspect of animal development is the appropriate polarisation of different cell types in the right place at the right time. Such polarisation is often precisely coordinated relative to the axes of a tissue or organ, but the mechanisms underlying this coordination are still poorly understood. Nevertheless, genetic analysis of animal development has revealed some of the pathways involved. Fo...

PURPOSE: We recently described a bioinspired material composed of nanoparticulate mineralized collagen glycosaminoglycan (MC-GAG) that induces efficient osteogenic differentiation of human mesenchymal stem cells (hMSCs) without the addition of exogenous growth factors, thereby suggestive of a promising, growth factorfree, materials-based method for clinically utilizable bone regeneration. We ha...

Wnts compromise a large family of secreted glycoproteins that have shown to be part of the signaling molecules that regulate several aspects of development such as axis formation and midbrain development [1, 2]. In mammals at least 19 Wnt members have been found. The interaction of a Wnt protein with members of the Frizzled (Fz) family of seven-pass trans‐ membrane cell-surface receptors trigge...

Coordinated morphogenetic cell movements during gastrulation are crucial for establishing embryonic axes in animals. Most recently, the non-canonical Wnt signaling cascade (PCP pathway) has been shown to regulate convergent extension movements in Xenopus and zebrafish. Heparan sulfate proteoglycans (HSPGs) are known as modulators of intercellular signaling, and are required for gastrulation mov...

Hedgehog(HH) pathway is found to be activated through a manner of canonical, or the non-canonical HH pathways. Distinct hyperplasia stroma around tumor cells is supposed to express pro-inflammatory cytokines abundantly, such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), etc. in pancreatic ductal adenocarcinoma (PDAC) tissues. In this study we observed the effects of TNF-α and I...

Wnt signaling plays critical biological roles during normal embryonic development andhomeostasis in adults. In the canonical pathway, binding of Wnt ligands to the Frizzled (Fzd) receptor and the low density lipoprotein-related receptor (LRP) 5 or LRP6 coreceptor initiates downstream signaling events leading to gene activation by -catenin and the T-cell factor (TCF)-lymphoid enhancer factor (LE...

Common intronic variants in the Human fat mass and obesity-associated gene (FTO) are found to be associated with an increased risk of obesity. Overexpression of FTO correlates with increased food intake and obesity, whilst loss-of-function results in lethality and severe developmental defects. Despite intense scientific discussions around the role of FTO in energy metabolism, the function of FT...

The Wnt/β-catenin signaling pathway is the main target of tooth regeneration regulation. Treatment cells with AZD2858 stimulates pathway, yet function this in remains unclear. Here, we found that promotes accumulation β-catenin nuclei stem from apical papilla (SCAPs) and enhances cell proliferation. Single-cell sequencing was performed on SCAPs treated AZD2858. Eight clusters were identified, n...