Catalytic nanomaterials, widely used as substitutes of peroxidase, exhibit unique properties, which are unattainable for native enzymes. However, their activity is usually examined by means of substrates developed and methods standardized for horseradish peroxidase (HRP). The aim of the presented work was to determine the scope of usefulness of chromogenic substrates for gold nanoparticle (AuNP...

LITERATURE is replete with references to the determination of serum cholesterol and its esters. This can probably be attributed to 2 hnain reasons : the difficulty in chemically determining this sterol in serum with any degree of specificity, and the present intense interest in the relationship of cholesterol to atherosclerosis. Several excellent reviews of cholesterol and methods for its deter...

AIMS To investigate the immunohistochemical expression of CD31 (JC70) in normal and neoplastic plasma cells. METHODS Plasma cells in bone marrow biopsies and extramedullary locations were examined. All extramedullary biopsies were formalin fixed and paraffin embedded. The bone marrow biopsies were fixed in formal acetic acid and embedded in paraffin wax. Twenty multiple myelomas (12 bone marr...

A healthy vaginal ecosystem has been shown to be protective against the acquisition of human immunodeficiency virus and gonorrhea, and women who are colonized with H(2)O(2)-producing lactobacilli are more likely to maintain a normal vaginal flora than women with lactobacilli that do not produce H(2)O(2). The purpose of this study was to formulate a testing medium that better supports the growth...

The red coloration produced by the interaction of an aromatic phenol and Millon’s reagent (mercuricnitrate indilutenitrous acid) is one of the most characteristic of the color reactions of the proteins. Among the nineteen known compounds which form the protein molecule only one, p-oxy-a-amino phenyl propionic acid (tyros’n) is of such a nature as to give a coloration with Millon’s reagent and t...

A very simple, rapid and cost effective spectrophotometric method is described for the determination of isoxsuprine hydrochloride (ISX). The method is based on the selective reduction of Folin-Ciocalteu’s reagent (F-C reagent) by phenolic and amino group present in ISX in alkaline medium to form a blue coloured chromogen which absorbs maximally at 770 nm. Beer’s law is obeyed in the concentrati...

In order to identify prolactin-producing tumours in human pituitary glands, 45 chromophobe adenomas, obtained from unselected necropsies, have been studied by various staining procedures including the immunoperoxidase technique for the demonstration of prolactin. The presence of immunoreactive prolactin was revealed in the cytoplasm of the tumour cells in six cases (13%), indicating that the oc...