Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at high spatial resolution with low detection limits. In contrast to the matrix-assisted laser desorption/ionization (MALDI-MS) approach, surface-assisted (SALDI) can be more effective in small molecules due absence interfering background signals m/z ranges. We developed functionalized TiO...

BACKGROUND Catharanthus roseus is an important Ayurvedic medication in traditional medicine. It is potentially used in countries like India, South Africa, China and Malaysia for the healing of diabetes mellitus. Although, the molecular mechanisms behind this effect are yet to be exclusively explored. Due to the great antidiabetic and hyperlipidemic potential of c. roseus, we hypothesized that t...

A procedure for cryogenic storage of alkaloid producing cell lines of periwinkle, Catharanthus roseus (L.) G. Don., has been developed. The procedure differs from established cryopreservation protocols in several aspects. Specifically, 4-day-old suspension subcultures of three cell lines were precultured in nutrient media supplemented with 1 molar sorbitol for 6 to 20 hours. The cells were then...

methods such as antisense–cosuppression technologies or overexpression, scientists could tailor medicinal plants to produce pharmaceutically important secondary metabolites by eliminating interfering metabolic steps or by introducing desired steps. For example, to accumulate more of an end product, analysts could block a side pathway that also uses the same precursor (6). A transgene could be i...