Human interferon prepared by challenge of leukocytes with Sendai virus, or of fibroblasts with double-stranded poly(inosinic acid).poly(cytidylic acid), has been studied with respect to purification by affinity chromatography. Both leukocyte and fibroblast interferons are removed from crude tissue culture fluids by means of columns of antibody to leukocyte interferon attached to Sepharose-4B. T...

We have found that the crude lymphokines, which prime the human monocyte-derived macrophage to generate H2O2 and exert microbicidal activity against intracellular Leishmania donovani, are rich in interferon (IFN)-gamma (600-3,000 U/ml). To determine the role of this specific lymphocyte product in macrophage activation, lymphokines were pretreated with a monoclonal antibody that neutralizes huma...

Triticum mosaic virus (TriMV), an economically important virus infecting wheat in the Great Plains region of the USA, is the type species of the Poacevirus genus in the family Potyviridae. Sensitive and high-throughput serology-based detection methods are crucial for the management of TriMV and germplasm screening in wheat breeding programs. In this study, TriMV coat protein (CP) was expressed ...

Supplementation increased the total dry matter intake by 18.2-36.8 %. Supplements given alone or in combination had no effect on dry matter and organic matter digestibility of the diet. Crude protein digestibility was decreased with molasses supplementation. Crude fiber digestibility was not affected by baker’s yeast supplementation but decreased with other supplements. The digestibility of nit...

On the Balkan Peninsula, sheep lamb during the winter months, which necessitate to house and feed the lambs in indoor production systems. The proper nutrition of growing lambs is essential for their growth and development during the winter month, and the aim is attaining the desired live weight over a short time period with low feed expenditure. During that period, the major part of the ration ...

Thrombosis and transmission of viral diseases are the principal adverse effects of current replacement therapy for factor IX deficiency when using heat-treated concentrates of vitamin K-dependent coagulation factors. More highly purified factor IX preparations could decrease the risk of disease transmission, reduce patient exposure to allogeneic proteins, and reduce the risk of thrombosis. In t...

Human T-cell growth factor (TCGF), a mitogenic protein that appears in the media of cultured lymphocytes after phytohemagglutinin-stimulation, has been purified more than 400-fold from serum-free conditioned media by using a sequence of ion exchange chromatography and gel filtration. The purified growth factor elutes as a broad peak from DEAE-Sepharose, focuses diffusely at a pH of about 6.8 on...

Despite improved awareness of work-related diseases and preventive measures, many workers are still at high risk of developing occupational hypersensitivity airway diseases. This stems from a lack of knowledge of bioaerosol composition and their potential effects on human health. Recently, archaea species were identified in bioaerosols, raising the possibility that they play a major role in exp...

Cardiolipin is a phospholipid and was isolated by Pangborn (1941, 1942) while investigating the nature of the antigenic material in beef heart muscle used in the Wassermann reaction. It was shown that cardiolipin was serologically active when used together with lecithin, and that the sensitivity of the reaction was enhanced by the addition of cholesterol. A monograph on cardiolipin antigen was ...