The selective suppression of the polymerase chain reaction and methods based upon it (construction ofcDNA libraries from low amounts of biological material, subtractive hybridization and differential display of mRNA, fast cloning of full-size cDNA, chromosome walking, cloning in vitro, and others) are reviewed. These methods display a high effectiveness and, taken together, enable intricate DNA...

PART I CLONING TECHNIQUES 1 Cloning Neuropeptide Tyrosine cDNA Carolyn A. Worby ......................................................................... 3 2 Human Y1/Y5 Receptor Gene Cluster: Isolation and Characterization Herbert Herzog............................................................................ 13 3 HumanType 2 Neuropeptide Y Receptor Gene: Isolation and Characterization Davi...

Subtractive cloning is a powerful technique for isolating genes expressed or present in one cell population but not in another. This method and a related one termed positive selection have their origins in nucleic acid reassociation techniques. We discuss the history of subtractive techniques, and fundamental information about the nucleic acid composition of cells that came out of reassociation...