Morphogenetic mechanisms such as cell movement or tissue separation depend on cell attachment and detachment processes, which involve adhesion receptors as well as the cortical cytoskeleton. The interplay between the two components is of stunning complexity. Most strikingly, the binding energy of adhesion molecules is usually too small for substantial cell-cell attachment, pointing to a main de...

Statins, hydroxymethylglutaryl-coenzyme-A reductase inhibitors (HMG-Co-A), are known to reduce plasma cholesterol levels. However, the biocompatibility of Simvastatin with human bone tissue has not been studied thoroughly. The purpose this study was further investigate effectiveness different concentrations simvastatin on attachment, proliferation, toxicity, cell cycle, and apoptosis normal ost...

In previous studies rat hepatocytes have been shown to adhere to substrates composed of collagen or fibronectin . In the present communication, the basement membrane protein laminin is reported to mediate the attachment and spreading of hepatocytes. The cell attachment-mediating activity of laminin was compared with that of fibronectin . The activity of fibronectin was heat sensitive, whereas l...

Cell entry by paramyxoviruses requires fusion of the viral envelope with the target cell membrane. Fusion is mediated by the viral fusion (F) glycoprotein and usually requires the aid of the attachment glycoprotein (G, H or HN, depending on the virus). Human respiratory syncytial virus F protein (F(RSV)) is able to mediate membrane fusion in the absence of the attachment G protein and is unique...

Cementum occupies a unique anatomical location where soft connective tissues of the periodontium are attached to root surfaces. Cell attachment properties of proteins present in cementum were studied. Human and bovine cementum were extracted with 0.5 mol/L CH3COOH followed by 4 mol/L guanidine, and proteins were separated by ion-exchange chromatography and SDS-polyacrylamide gel electrophoresis...

Attachment and penetration of influenza virus into clone 1-5C-4 cells were quantitatively determined by the immunofluorescent cell-counting assay. Aided by centrifugal force, more than 95% of virus inocula of five representative influenza virus strains (A(0)/PR8, A(1)/Ann Arbor, A(2)/Japan, B/Lee, B/Great Lakes) were attached to cells at a linear rate within 10 min, in contrast to approximately...

Kinetochores direct attachment of chromosomes to microtubules of the mitotic spindle during cell division. Three recent studies in Cell, including one in this issue, reveal important new roles for two kinetochore protein complexes-Ndc80 and INCENP-Survivin-in establishing the correct attachment of chromosomes to spindle microtubules (Cheeseman et al., 2006, DeLuca et al., 2006 and Sandall et al...

Quantitative information about adhesion strength is a fundamental part of our understanding of cell-extracellular matrix (ECM) interactions. Adhesion assays should measure integrin-ECM bond strength, but reports now suggest that cell components remain behind after exposure to acute force for radial shear assays in the presence of divalent cations that increase integrin-ECM affinity. Here, we sh...

     In this research, hydrogels based on poly vinyl alcohol and poly acrylic acid blend were prepared which were cross-linked by applied thermal conditions. Afterward, effects of time and heating on water uptake were investigated. The highest water uptake value exhibited by the sample that was heated for 20 min. at 110 ºC was about 2129% after 4 days at equilibrium state. Hydrogels exhibited p...

PURPOSE In general, both chemical composition and surface features of implants affect cell response. The aim of this study was to evaluate the effect of titanium (Ti) passivation on the response of rat bone marrow cells, considering cell attachment, cell morphology, cell proliferation, total protein content, alkaline phosphatase (ALP) activity, and bonelike nodule formation. MATERIALS AND MET...