A hybrid simulation framework was proposed to predict the dynamics in cell culture bioreactors. The model is based on a multi-agent approach where CHO cells are considered as individuals (agents) following a rule base governing their behavior, while a flux balance model is embedded in agents to predict quantitative changes in nutrient and metabolite concentrations. The model takes the measured ...

Background Specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (ESCs) differentiation. In this study,the effects of bone morphogenetic protein 4 (BMP4) and mouse embryonic fibroblasts (MEFs) co-culture system on germ cell differentiation from mouse ESCs were studied. MaterialsAndMethods Cell suspension was prepared from one-day-old embryoid bo...

Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...

Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey. In this Cell Culture, we explore cellular and molecular mechanisms used by pr...

On June 11 th , the best soccer players from around the world will meet in South Africa at the FIFA 2010 World Cup. To reach this level of competition, these players combine phenomenal ball-handling skills with three major characteristics: fast sprinting, rapid reaction times, and ferocious tenacity. This issue's Cell Culture explores these three athletic qualities from a molecular and cellular...

Cell cultures are indispensable to develop and study efficacy of therapeutic agents, prior to their use in animal models. We have the unique ability to model well differentiated human airway epithelium and heart muscle cells. This could be an invaluable tool to study the deleterious effects of toxic inhaled chemicals, such as chlorine, that can normally interact with the cell surfaces, and form...

: Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. Errors of transferred models in the declining pha...