The rise in world industrialization and the cost of importing enzyme by local industries have led to arise search for novel native producing microorganisms. Cellulase is an that catalyzes breaking down carbon chains cellulose hemicellulose, this research therefore aimed at studying fungal cellulase produced Penicillium expansum grown on malus domestica (apple fruits). Fresh apple fruit was allo...

Clostridium acetobutylicum NRRL B527 and ATCC 824 exhibited extracellular and cell-bound endoglucanase and cellobiase activities during growth in a chemically defined medium with cellobiose as the sole source of carbohydrate. For both strains, the endoglucanase was found to be mainly extracellular (70 to 90%) during growth in continuous or batch cultures with the pH maintained at 5.2, whereas t...

Cellulolytic enzymes are synthesized by a large number of micro-organisms which include fungi, actinomycetes, gliding bacteria (myxobacteria) and true bacteria. However, only the fungi appear to excrete large amounts of cellulase enzymes in active form into culture media and not surprisingly these are the enzyme systems that have been most extensively studied. Bacterial cellulases are cell-wall...

Eight isolates of cellulose-degrading bacteria (CDB) were isolated from four different invertebrates (termite, snail, caterpillar, and bookworm) by enriching the basal culture medium with filter paper as substrate for cellulose degradation. To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were me...

Nine aerobic cellulolytic bacterial cultures were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). The objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (SSF) of palm kernel cake (PKC). The ba...

Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionizatio...

Nine thermophilic cellulolytic clostridial isolates and four other noncellulolytic bacterial isolates were isolated from self-heated biocompost via preliminary enrichment culture on microcrystalline cellulose. All cellulolytic isolates grew vigorously on cellulose, with the formation of either ethanol and acetate or acetate and formate as principal fermentation products as well as lactate and g...

Cellulase is the key enzyme in the bio refinery process of producing green chemicals. As cellulase production cost is the major factor in these processes, there is lot of need to isolate the effective producers of cellulase from the natural environment which can degrade the abundantly available lignocellulose materials. Congo red clearing zone assay, gel diffusion assay and dyed congo red filte...

The constraints and advantages in cellulolytic enzymes production by fungal biofilms for a consolidated bioconversion process were investigated during this study. The biofilm cultivations were carried out in reactors designed for consolidated bioprocessing Multispecies Biofilm Membrane reactors, (MBM) where an aerobic fungal biofilm produces the lignocellulolytic enzymes while a fermenting micr...