recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

For the first time, from early 2019 to end of 2022, Mars’ shallow and deep interiors have been explored by seismology with InSight mission. Thanks performances its seismometers quality their robotic installation on ground, 1,319 seismic events detected, including about 90 marsquakes at teleseismic distances, Mw 2.5 4.7 least 6 impacts, largest ones craters larger than 130 m. A large fraction th...

BACKGROUND Cystine-knot (cys-knot) structure is found in a rather large number of secreted proteins and glycoproteins belonging to the TGFbeta and glycoprotein hormone (GPH) superfamilies, many of which are involved in endocrine control of reproduction. In these molecules, the cys-knot is formed by a disulfide (SS) bridge penetrating a ring formed by 8, 9 or 10 amino-acid residues among which f...

The regeneration of bone is a remarkable, complex physiological process, and BMPs are a formidable clinical tool to promote its regeneration. By defining roles played by BMPs in developmental biology and bone regeneration, significant progress has been made to identify cell-signaling molecules and their regulators. For example, the regulators of BMPs that include noggin, chordin, cerberus, dan,...

The facial neural crest (FNC), a pluripotent embryonic structure forming craniofacial structures, controls the activity of brain organisers and stimulates cerebrum growth. To understand how the FNC conveys its trophic effect, we have studied the role of Smad1, which encodes an intracellular transducer, to which multiple signalling pathways converge, in the regulation of Foxg1. Foxg1 is a transc...

Volcanic activity on Mars peaked during the Noachian and Hesperian periods but has continued since then in isolated locales. Elysium Planitia hosts numerous young, fissure-fed flood lavas with ages ranging from approximately 500 to 2.5 million years (Ma). We present evidence for what may be youngest volcanic deposit yet documented Mars: a low albedo, high thermal inertia, high-calcium pyroxene-...