BACKGROUND The human kallikrein 10 gene [KLK10, also known as normal epithelial cell-specific 1 gene (NES1)] is a member of the human kallikrein gene family. The KLK10 gene encodes for a secreted serine protease (hK10). We hypothesize that hK10 is secreted into various biological fluids and that its concentration changes in some disease states. The aim of this study was to develop a sensitive a...

In earlier communications (1, 2) there was described a rapid method for diagnosing meningococcal meningitis and ascertaining the serological type of the organism by means of precipitin tests carried out on the cerebrospinal fluid with monovalent antisera. I t was pointed out that a certain number of negative results occurred when the spinal fluid tested was drawn after the commencement of intra...

background: the prevalence of setariasis in cattle and cerebrospinal nematodiasis in sheep and goats were determined in two geographical regions of iran. methods: in two provinces of iran: mazandaran (zone i) and qazvine (zoneii)   where sheep and goats were suffering from symptoms similar to cerebrospinal nematodiasis, the peritoneal cavities of  763 and 1020 cattle were searched for adult set...

Advances in technology, especially in molecular biology, allow for a fast expansion of diagnostic methods in routine clinical practice. New proteomics and genomics technologies could be used for disease specific biomarker discovery and to monitor patient response to the therapy. Genomics and proteomics may also help to establish new, molecular classification of the disease. Applying genomic and...

The MB/BacT automated system is designed for the isolation of mycobacteria from clinical specimens. It utilizes a colorimetric sensor and reflected light to continuously monitor the CO2 concentration in the culture medium. We compared its performance to that of the BACTEC 12B media for the radiometric BACTEC 460 instrument and that of solid culture media. Respiratory specimens and urine samples...

The sensitivity of PCR-based methods for the detection of DNA offers opportunities for tumor diagnosis from the small amounts of tumor-derived DNA released into body fluids. We report the detection of tumor DNA in the cerebrospinal fluid (CSF) of two patients with intracranial neoplasms. One patient had a metastatic breast carcinoma which contained amplified HER-2/neu genes, and amplified HER-2...

Amyloid β-peptide (Aβ) in its oligomeric form is often considered as the most toxic species in Alzheimer's disease (AD), and thus Aβ oligomer is a potentially promising candidate biomarker for AD diagnosis. The development of a sensitive and reliable method for monitoring the Aβ oligomer levels in body fluids is an urgent requirement in order to predict the severity and progression at early or ...