A protease can be defined as an enzyme capable of hydrolyzing peptide bonds. Thus, characterization of a protease involves identification of target peptide sequences, measurement of activities toward these sequences, and determination of kinetic parameters. Biological protease substrates based on fluorescent protein pairs, which allow for use of fluorescence resonance energy transfer (FRET), ha...

We combine optical methods for sensing of oil films. These are: coherent fringe projection (CFP), digital holographic in-line interferometry (DHILI). The methods of CFP and DHILI are described as coherent superposition of partial interference patterns OCIS codes; 090.0050, 090.199,120.2880

The main purpose of this paper is to establish general conditions under which T2-spaces are compact-covering images of metric spaces by using the concept of cfpcovers. We generalize a series of results on compact-covering open images and sequencecovering quotient images of metric spaces, and correct some mapping characterizations of g-metrizable spaces by compact-covering σ-maps and mssc-maps.

Increasing interest in sustainability performance (environmental, social, and governance pillar [ESGP]) corporate financial (CFP) is noteworthy. However, we do not find any all-inclusive study that employs both individual components of environmental, pillars (ESG) as well the cumulative ESG score on accounting market firms. Furthermore, puts forth “best practices” ESGP-CFP nexus. Therefore, our...

The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between three proteins microscopically. Flow cytometry offers excellent sensitivity, effective signal separation and the capacity to assess a large number of events, and, therefore, should be an ideal means to explore p...

BACKGROUND Discriminating latent tuberculosis infection (LTBI) from active TBI may be challenging. The objective of this study was to produce the recombinant L-alanine dehydrogenase (AlaDH) antigen and evaluate individuals with LTBI, those with active TBI, and uninfected individuals by enzyme-linked immunospot assay (ELISPOT) in order to distinguish LTBI from active TBI. METHODS This explorat...

The sudden increase in information derived from the completed Mycobacterium tuberculosis (Mtb) genome sequences has revealed the need for approaches capable of converting raw genome sequence data into functional information. To date, an experimental system for studying protein-protein association in mycobacteria is not available. We have developed a simple system, termed mycobacterial protein f...

In this paper, we define the crowdfunding platform (CFP) a virtual place where project creators document their projects for gaining awareness or social support for making the project proceed. By doing so, project teams can voice the propositions they have worked on or they may also speak out on one party’s behalf for seeking advices. This paper proposes a research framework that targets the iss...