Previous investigators have grown or maintained adult or embryonic skin in tissue culture and on the chorioallantoic membrane (CAM) of chickens. These studies have revealed two important factors involved in culturing skin: ( 1) the presence of an inducer which originates in the dermis and affects the phenotype of epithelial cells; and (2} the influence of ·~he physical surface of the substrate ...

PURPOSE We describe the use of chick chorioallantoic membrane (CAM) as a model system for the study of the precision and safety of vitreoretinal microsurgical instruments and techniques. METHODS The CAM was prepared for experimentation with and without its inner shell membrane (ISM) attached for in vivo and in vitro experiments that simulated medical and surgical interventions on the retina. ...

Antiangiogenesis, e.g., inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists.The chick embryo chorioallantoic membrane ...

Angiogenesis provides nutrients and oxygen to promote tumor growth and affords a channel that facilitates tumor cell entry into the circulation. Andrographolide (Andro) possess anti-tumor activity; however, its direct effect on angiogenesis still needs to be clarified. In this study, our experiments revealed that Andro significantly inhibited vascular growth in chick embryo chorioallantoic memb...

Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumorogenic, anti-infl ammatory and anti-angiogenic. For the latter, varieties of angiogenesis factors can suppress this issu...

During development, the chick embryo mobilizes the calcium it needs from two extraembryonic sources, first the yolk and then the eggshell. Since previous studies have strongly suggested that vitamins D and K may regulate chick embryonic calcium metabolism, we have examined here how these vitamins might be involved in regulating the calcium mobilization processes. We used as our experimental sys...

The formation of membrane bone from neural crest-derived mesenchyme of the maxillary and mandibular processes of the embryonic chick depends upon prior interactions between the mesenchyme and maxillary or mandibular epithelia. The present study explores the specificity of these interactions using tissue recombinations between heterotypic epithelia and mesenchyme. Mandibular and maxillary mesenc...