We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and the deacetylation of histone H3 Lys 4 (H3K4) at the centromere. HDAC3 knockdown induced spindle assembly checkpoint activation and sister chromatid dissociation. The depletion of Polo-like kinase 1 (Plk1) or Aurora B restored cohesion in HDAC3-depleted cells. HDAC3 was also required for Shugoshin localiz...

BACKGROUND Implant breakage after the fixation of traumatic fractures is rare; however, when it occurs, it is debilitating for the patients and a challenge for surgeons. The purpose of this study was to analyze and identify the independent risk factors for implant breakage of traumatic fractures treated with plate osteosynthesis. METHODS We reviewed the medical records of patients with a frac...

the rotor of enegelberg whitener was equipped with a screw conveyor to reduce broken rice percentage. according to preliminary experiments this modification could reduce broken rice percentage about 2.5%. further experiment was performed to determine the effect of rotor speeds and blade positions on white rice quality. to do this, five rotor speeds, consisting 500, 600, 700, 800 and 900 r.p.m a...

Composite or closely linked maize (Zea mays) Ac/Ds transposable elements can induce chromosome breakage, but the precise configurations of Ac/Ds elements that can lead to chromosome breakage are not completely defined. Here, we determined the structures and chromosome breakage properties of 15 maize p1 alleles: each allele contains a fixed fractured Ac (fAc) element and a closely linked full-le...

Plates of austenitic stainless steel are often used in orthopedic surgery for fixing ends of the broken bones and for repairing fractures. The paper analyzes a premature breakage of six plates for fixing broken bones with different patients during their recovery. Each plate's breakage can induce serious aftermaths such as a new surgery, unexpected undesired complications and a prolonged healing...

Detection of tool breakage is very important for automated machining operations. This paper presents a negative-selection algorithm for tool breakage detection. The method is inspired by the defense mechanism of the immune system, which discriminates between self and non-self. Here self is deened to be normal cutting operations and non-self is any deviation beyond allowable variation of the cut...

Poly(ADP-ribose) polymerases (PARPs) regulate various aspects of cellular function including mitotic progression. Although PARP inhibitors have been undergoing various clinical trials and the PARP1/2 inhibitor olaparib was approved as monotherapy for BRCA-mutated ovarian cancer, their mode of action in killing tumour cells is not fully understood. We investigated the effect of PARP inhibition o...

Sister chromatid cohesion is essential for cell viability. We have isolated a novel temperature-sensitive lethal mutant named eso1-H17 that displays spindle assembly checkpoint-dependent mitotic delay and abnormal chromosome segregation. At the permissive temperature, the eso1-H17 mutant shows mild sensitivity to UV irradiation and DNA-damaging chemicals. At the nonpermissive temperature, the m...

To investigate whether the fragile X syndrome is associated with a generalised chromosomal instability, we compared the frequency and distribution of chromosomal breakage in lymphocytes grown in low folate medium from normal subjects and from patients with the syndrome. Although low folate conditions increased the rate of chromosome breakage, no difference in frequency or distribution of chromo...