Two isolates of serum-susceptible Campylobacter coli were recovered in a 7-day interval from blood from a patient with hepatocellular carcinoma and liver cirrhosis whose peritoneal-caval (Denver's) shunt malfunctioned. Identical random amplified polymorphic DNA fingerprints, cellular fatty acid chromatograms, and antibiograms of the two isolates indicate that C. coli has the ability to cause ca...

This Application Note describes the development of conventional HPLC and Rapid Resolution LC (RRLC) methods for the analysis of Buplerui (Chaihu). A comparison of fingerprint chromatograms of different Buplerui samples showed different peak profiles of different TCMs collected from different places. The method for the Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) was optimize...

We report here a simple method of directly visualizing in automated DNA sequencing chromatograms DNA methylations of different types including cytosine methylations in Hpa II and dcm sites as well as adenine methylations in dam sites. This is made possible by the observation that the extent of incorporation of fluorescently labeled dideoxynucleotides is influenced by the methylated bases in tem...

Substances with pharmacological properties similar to those of serotonin (5-hydroxytryptamine) have been found in human and dog urine and in rat, rabbit, and dog brain by Twarog and Page (1). Eluates of chromatograms of urine showed greatest activity from areas having the same RF value as serotonin. Since then, these eluates have been rechromatographed and other positive indole spots have appea...

Justicia adhatoda L. which belongs to the family of Acanthaceae contains alkaloids like vasicine, vasicinone and deoxyvasicine. These alkaloids give the plant its expectorant activity, antispasmodic, antiseptic and antihelmintic properties. Hence, in the present investigation attempts were made to isolate and characterise alkaloids from callus and suspension cultures of J. adhatoda. Alkaloids w...

Simple and rapid thin-layer chromatographic methods, using heel or finger-tip blood, are describedthat are suitable for screening for inborn errors of amino acid metabolism. Serum is chromatographed without deproteinizing. A variation of this method utilizes blood impregnated on paper discs for chromatography. These methods are much faster, more sensitive, and provide better resolutionof amino ...

The type 2 fimbrial lectin of Actinomyces naeslundii WVU45 mediated the binding of this bacterium to glycosphingolipids chromatographed on thin-layer silica gel plates. Radioiodinated bacteria attached to GM1, GD1b, and globoside. After chromatograms were treated with sialidase, the bacteria also bound to GD1a and GT1b. The actinomyces lectin apparently recognized the Gal beta 3GalNAc termini o...