Microbial analyses of fermented milk products require selective methods to discriminate between close species simultaneously present in high amounts. A culture-based method combining novel chromogenic agar media and appropriate incubation conditions was developed to enumerate lactic acid bacteria (LAB) strains in fermented milk. M1 agar, containing two chromogenic substrates, allowed selective ...

OBJECTIVE To investigate if introital and vaginal flushing samples inoculated on chromogenic agar could increase the recovery rate and rapid identification of Candida and non-albicans species, as compared to culture of posterior vaginal fornix samples on Sabouraud agar and speciation of isolates by biochemical tests. METHODS Samples from the introitus and the posterior vaginal fornix and vagi...

Chelidonium majus is a well-known source of biologically active compounds. Most them are alkaloids, which used in researches and for tradition medicine. In this study, we explored the influence C. crude total extract onto blood coagulation system vitro, primary secondary hemostasis. Aim. To study process. Methods. Turbidimetry plasma, APTT test with chromogenic substrates, platelet aggregation ...

cholera outbreak is more common in developing countries. the causative agent of the disease is vibrio cholerae strains o1 and o139. traditional diagnostic testing for vibrio is not always reliable, because vibrio can enter a viable but non cultivable state. therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       in this investigation, a ...

BACKGROUND Screening at hospital admission for carriage of methicillin-resistant Staphylococcus aureus (MRSA) has been proposed as a strategy to reduce nosocomial infections. The objective of this study was to determine the long-term costs and health benefits of selective and universal screening for MRSA at hospital admission, using both PCR-based and chromogenic media-based tests in various se...

This study was performed to investigate the use of chromogenic tube and methyl blue-Sabouraud agar for the presumptive identification of Candida albicans. 124 clinical isolates, including 111 C.albicans and 13 Candida spp strains, which had been identified by morphology on cornmeal tween 80 agar and Vitek automated identification system, were included. Three different identification procedures,...

Electrostatic interactions during activation of coagulation factor X were analysed by comparing effects of ionic strength on reaction rates with predictions of classical electrostatic theory. Geometrical correlations were investigated using alpha-shape-based computations on the crystal structure of Ca-fragment 1 of prothrombin. The ionic strength of the reaction environment was controlled with ...

OBJECTIVE Intravitreal fibrin formation is a frequent observation after vitrectomy performed for a variety of vitreoretinal disorders including proliferative vitreoretinopathy (PVR), proliferative diabetic retinopathy (PDR), and endophthalmitis. Plasminogen activators (PA) have been used for the management of this postoperative complication. This approach requires the presence of plasminogen, t...