BACKGROUND Many studies have used creatinine kinase (CK), myocardial bound for CK (CK-MB), and cardiac troponin I (cTnI) and T (cTnT) to evaluate myocardial cells injury after ablation. We applied measurements of the blood concentration of cardio-specific biomarkers as surrogates for the injured cell mass. AIM To clarify which of the standard biomarkers are useful in the evaluation and quanti...

We examined the clinical and analytical performance of two immunoassays (Becton Dickinson CK-MB; Ciba-Corning Magic Lite CK-MB) in which monoclonal anti-CK-MB antibodies are used for directly measuring creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB) in serum, and also one electrophoretic method (Ciba-Corning). Within- and between-assay precision for both immunoassays was good at the upper ref...

The rates of degradation of creatine kinase subunits, M-CK and B-CK subunits, were measured in cultured myogenic cells and in subcultured fibroblasts. In differentiated myogenic cells, the myotubes, both M-CK and B-CK subunits are synthesized. Their rates of degradation were compared. The M-CK subunits is slightly more stable and is degraded with an average apparent half-life of 75 h, whereas t...

Gutjahr, Welzl and Woeginger found polynomial-time algorithms for a number of digraph homomorphism problems. These algorithms are based on the X-enumeration, the Ck-extended X-enumeration and the X-graft construction. In this note we show how the last two methods can be combined to obtain new polynomial-time algorithms, which also work for list homomorphisms. In the process we are able to exten...

It is not uncommon for a rheumatologist to get a request for evaluating a patient with persistent elevation of serum creatine (phospho) kinase (CK) level (hyper-CK-emia) especially if this laboratory abnormality is persistent. We present a descriptive analysis of 16 consecutive adult patients seen with a condition called idiopathic benign hyper-CK-emia at a tertiary care Rheumatology Clinic in ...

Two new pyrene-cored covalent organic polymers (COPs), CK-COP-1 and CK-COP-2, were synthesized via the one-step polymerization of two thiophene-based isomers, 1,3,6,8-tetra(thiophene-2-yl) pyrene (L1 ) and 1,3,6,8-tetra(thiophene-3-yl) pyrene (L2 ). The resulting pyrene-cored COPs exhibit rather different surface areas of 54 m2 g-1 and 615 m2g-1 for CK-COP-1 and CK-COP-2, respectively. The CO2 ...

We compared the usefulness of four serum assays for classifying patients originally suspected of having an acute myocardial infarction. One of these is the long-used measurement of total creatine kinase (CK) activity. The other three are relatively new immunoassays: myoglobin by RIA, CK-BB by RIA, and CK-MB by immunoinhibition. When we evaluated test effectiveness with use of conventionally der...

BACKGROUND Serum creatine kinase-MB isoenzyme (CK-MB) is widely used as a marker of myocardial injury. We prepared recombinant human CK (r-hCK) MB isoenzyme and examined its potential for use as a control material for assay of CK-MB in serum. METHODS cDNAs encoding CK-M and CK-B subunits were inserted into the same plasmid vector, followed by transformation of Escherichia coli. The resulting ...

CK-MB values of >9 U/L and >4% CK-MB for potential MI. Because the modified calibration causes an “apparent” loss of CK-MB at the low end when compared with the KOdak calibration, we needed to determine whether the modified procedure retained good sensitivity. We analyzed a series of samples from two patients who displayed clinical evidence of MI 24 to 48 h before admission. The Hybritech proce...