Staphylococci are known as clustering Gram-positive cocci, nonmotile, non-spore forming facultatively anaerobic that classified in two main groups, coagulase-positive and coagulase-negative. Staphylococcus epidermidis with the highest percentage has the prominent role among coagulase-negative Staphylococci that is the most important reason of clinical infections. Due to various virulence factor...

The plate test, a modification of the slide test described by Cadness-Graves was developed for the rapid identification of coagulase-positive staphylococci in conjunction with bacteriophage typing. An evaluation of 1,145 cultures by three coagulase-determination methods, the slide, tube, and plate tests, indicates that the plate test is as accurate as the slide tests, and the plate test agrees ...

The incidence of infections due to coagulase-negative staphylococci (CoNS) has been steadily increasing in recent years. In this report, we describe an isolate of the CoNS Staphylococcus lugdunensis from a wound specimen with a colony morphology distinct from that of a classical S. lugdunensis colony. Specifically, wrinkled, medium-sized, beta hemolytic, opaque, rough white colonies were detect...

A group of strains of Staphylococcus albus is described which produced neither coagulase nor haemolysin, was slightly sensitive or resistant to novobiocin, and sensitive to all other antibiotics, to sulphonamides, and to nitrofurantoin. The agglutinating antigen 51 was isolated from all strains from patients with urinary infections and abundant pyuria. In more than 40 cases studied it was not p...

Methodological modifications, particularly the use of different fixatives, may account for discrepancies between studies of the relation between virulence and biofilm production in vitro by isolates of coagulase negative staphylococci. The efficacy of formalin and Bouin's reagent for fixing coagulase negative staphylococcal biofilms in a microtitre tray assay was compared. The optical density o...

Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Fibrinogen clotting and fibrino-peptide formation by staphylocoagulase and the coagulase-reacting factor. J. Bacteriol. 85:628-635. 1963.-The mechanism of fibrinogen clotting by staphylocoagulase and the coagulase-reacting factor (CRF) was studied from the standpoint of the products of the reaction, and compared with throm...

Preliminary experiments revealed that coagulase positive staphylococci could be distinguished culturallv from coagulase negative staphylococci in a soft agar medium containing a small amount of normal human or rabbit plasma. In a medium of suitably low viscosity, staphylococci produce elongated diffuse feathery colonies. When coagulable plasma is incorporated in such a medium, the colonies of c...

Vancomycin resistance has been reported in clinical isolates of both coagulase-negative staphylococci and Staphylococcus aureus. The emerging threat of widespread vancomycin resistance poses a serious public health concern given the fact that vancomycin has long been the preferred treatment of antibiotic-resistant gram-positive organisms. Though major efforts are now being focused on improving ...

Eighty clinical specimens of BACTEC 9240 blood culture vials, culture positive for staphylococci (38 Staphylococcus aureus and 42 coagulase negative staphylococci), were tested directly for the presence of clumping factor/protein A and free coagulase. Seven commercial slide agglutination kits were compared with a direct-tube coagulase (DTC) method. All tests were performed on blood culture pell...

The detection of methicillin resistance was examined in 51 strains of Staphylococcus aureus and 135 strains of coagulase negative staphylococci using Isosensitest, Diagnostic Sensitivity Test (DST), Mueller-Hinton (MH), Columbia, and Sensitest agars. MH agar with 5% added sodium chloride incubated at 35 degrees C was the most effective in detecting resistance in S aureus, and Columbia agar with...