BACKGROUND Staphylococcus aureus strains are now regarded as zoonotic agents. In pastoral settings where human-animal interaction is intimate, multi-drug resistant microorganisms have become an emerging zoonotic issue of public health concern. The study of S. aureus prevalence, antimicrobial resistance and clonal lineages in humans, animals and food in African settings has great relevance, taki...

Staphylococcus lugdunensis is an emergent virulent coagulase-negative staphylococcus responsible for severe infections similar to those caused by Staphylococcus aureus. To understand its potentially pathogenic capacity and have further detailed knowledge of the molecular traits of this organism, 93 isolates from various geographic origins were analyzed by multi-virulence-locus sequence typing (...

Staphylococcus epidermidis is the most important member of coagulase negative staphylococci responsible for community and hospital acquired infections. Most clinical isolates of S. epidermidis are resistant to methicillin making these infections difficult to treat. In this study, correlation of methicillin resistance phenotype was compared with methicillin resistance (mecA) gene carriage in 55 ...

background:   methicillin-resistant   staphylococcus   aureus  (mrsa)   is  a major cause of nosocomial and community infections that are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. moreover panton-valentine  leukocidin (pvl) is an important virulence factor in s. aureus and causes white blood cell destruction, necrosis and  accelerat...

The present study was aimed to identify the frequency of coagulase (Coa) and thermonuclease (Nuc) genes and Staphylococcal enterotoxin A (Sea) production among Staphylococcus aureus isolated from various sources in Shiraz. Moreover, the correlation between the Sea gene and coagulase and thermonuclease enzymes is also considered. A total of 100 S. aureus were isolated from various sources includ...

The prevalence of methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitiveStaphylococcus aureus (MSSA) carriage among poultry and poultry farmers in Malaysia is largely unknown.In the current investigation, chickens and chicken farmers from 30 chicken farms were screened for MRSAand S. aureus carriage. The genetic characteristics of the isolates were determined through multi...

background: killer cell immunoglobulin-like receptors (kir) are expressed on nk cells and a subset of t cells. the variable kir receptors along with their ligands, hla class i, influence risk for autoimmune and malignant diseases. objective: to investigate the kir gene profiles in relation to susceptibility to graves’ disease in patients with ophthalmopathy. methods: kir genes profiles were ana...

the last two decades have seen a massive growth in the application of dna technology in histocompatibility and immunogenetics. this review summarises the history and application of dna methods in this area.

The usefulness of the API-STAPH system and a method based on ribosomal fingerprinting was evaluated by studying 89 coagulase-negative staphylococci isolated from goat milk. The bacteria were supposed to belong to the species Staphylococcus caprae. The results obtained from the API-STAPH system showed variations in their phenotypic features. DNA cleavage with EcoRI yielded six ribotypes and thre...

the aim of the study was to determine the resistance patterns of pseudomonas aeruginosa isolates recovered from patients with surgical wounds in hospitals and also to investigate their epidemiological relatedness using molecular typing techniques. twenty pseudomonas sp. isolated from surgical wounds were subjected to antibiotic susceptibility testing by disk diffusion, plasmid profile, sds-page...