The identification of cancer stem cell (CSC) populations in virtually all tumor types has widespread clinical consequences. CSCs are suggested to be the only cells within malignancies endowed with tumorigenic capacity and are, therefore, directly implicated in therapy resistance and minimal residual disease. The genetic and molecular mechanisms sustaining CSCs are only currently emerging. For i...

The present study is the development of colon targeted matrix tablets of the drug flurbiprofen, a NSAIDS of the class of Ibuprofen designed to prolonged the release for sustained effect. Different formulation (F1 TO F9) batches were made with the help of different polymers and their different proportions (Guar Gum, Eudragit RL, Eudragit RS ) with the help of Wet granulation techniques. The prep...

Colon cancer is one of the most common cancers in the world. Overexpression of MDR1 mRNA and P-gp is associated with the classic multidrug resistance of colon cancer cells. In our previous study, we reported on the transient specific reversal of MDR1/P-gp-dependent multi-drug resistance by RNA interference (RNAi) in colon cancer cells. In this study, RNAi targeting the MDR1 gene stably reversed...

Mucin 2 (MUC2) is the major secreted mucin of the large intestine and is expressed by adenomas and mucinous carcinomas. Since colon cancer is associated with a proinflammatory microenvironment and dysregulated MUC2 expression, the aim of this study was to characterize the effects of MUC2 gene expression in colon tumor progression using colonic cancer cells. CT26 colon cancer cells were stably t...

Increased polyamine synthesis and inflammation have long been associated with colon carcinogenesis in both preclinical models and in humans. Recent experimental studies suggest that polyamines may be mechanistically involved in colonic inflammatory processes. Genetic epidemiology results indicate that a single nucleotide polymorphism influencing the expression of a polyamine biosynthetic gene i...

The insulin receptor substrate-1 (IRS-1), a docking protein for both the type 1 insulin-like growth factor receptor (IGF-IR) and the insulin receptor, is known to send a mitogenic, anti-apoptotic, and anti-differentiation signal. Several micro RNAs (miRs) are suggested by the data base as possible candidates for targeting IRS-1. We show here that one of the miRs predicted by the data base, miR1...

Epidermal Growth Factor Receptor (EGFR) activates a robust signalling network to which colon cancer tumours often become addicted. Cetuximab, one of the monoclonal antibodies targeting this pathway, is employed to treat patients with colorectal cancer. However, many patients are intrinsically refractory to this treatment, and those who respond develop secondary resistance along time. Mechanisms...

As a pH-sensitive nanomaterial, Eudragit S100 has good colon targeting. However, little research been carried out on its mesoscopic scale. In this paper, the self-assembly behavior of Pulsatilla saponins D (PSD) and S100, as well loading release mechanism PSD, was investigated via computer simulations. The effects characteristics PSD in dry powder state drug-carrier ratio were explored by coars...

Klotho is a transmembrane protein which deficiency has pleiotropic effects in number of aging-related disease processes and various cancers. In some recent studies about klotho disorders, it been shown to effective cancers including lung, liver, breast, kidney, colon. TNF-related apoptosis-inducing ligand (TRAIL), TNF family molecule, cytokine that stimulates apoptosis through death receptors m...

Abstract Background Colorectal cancer is the third most prevailing in whole world. Chemotherapeutic agents which are used for treatment have severe side effects and also unwanted exposure to healthy cells. In present study, polymeric nanoparticles of SN-38 were prepared (using cationic anionic polymers). They optimized by Box Behnken design characterized its physicochemical properties vitro dru...