The erythroid colon-forming technique has been used to study the erythropoietin (Ep) sensitivity of CFU-E during ontogenesis. Evidence is provided which indicates that an age-related decreasing Ep sensitivity of CFU-E occurs as the animal develops. In addition, an intermediate Ep-sensitive CFU-E population represented by the 2-day-old neonate is present during the transition from foetus to adul...

The aim of this study was to evaluate, at a laboratory scale, the ability of this microorganism to grow in seawater and bioaccumulate in mussels (Mytilus galloprovincialis) maintained in constantly aerated tanks, containing twenty litres of artificial seawater. Three concentrations of A. butzleri LMG 10828(T) were tested (about 5 × 10⁶ CFU/mL, 5 × 10⁴ CFU/mL, and 5 × 10² CFU/mL). Following cont...

In this work the electrochemical disinfection of the effluent of a secondary wastewater treatment plant is investigated. In the experimental work, performed on-site with real effluents of the WWTP located in Vuelta Ostrera (Cantabria, Spain), boron-doped diamond (BDD) electrodes were employed. The initial concentration of E. coli in the effluent of the WWTP varied in the range 1.3 x 10⁴-5.2 x 1...

This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with ...

Olive mill wastewater polyphenol extract was tested for antimicrobial activity against 64 strains of Pseudomonas fluorescens responsible for mozzarella discolouration. The extract showed a minimum inhibitory concentration (MIC)50 value of 5 mg/mL and a MIC90 value of 7 mg/mL. The MBC50 and MBC90 values corresponded to 6 and 8 mg/mL, respectively. The MIC concentration (7 mg/mL) was demonstrated...

Monoacetyldiglycerides purified from deer antler, and identical synthetic compounds, have been shown to stimulate hematopoiesis. In the present study, we synthesized eight monoacetyldiglycerides, one of which, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (3), was structurally identical to one of the naturally occurring monoacetyldiglycerides and showed the most potent stimulation of hematopoie...

Several lines of evidence indicate that macrophage inflammatory protein-1alpha (MIP-1alpha) modulates the proliferation of hematopoietic progenitor cells, depending on their maturational stages. To clarify the mechanisms for the modulation of hematopoiesis by this chemokine, we examined the expression of a receptor for MIP-1alpha, CCR1, on bone marrow cells of normal individuals using a specifi...

BACKGROUND Hemorrhagic shock (HS) is the major leading cause of death after trauma. Up to 50% of early deaths are due to massive hemorrhage. Excessive release of pro-inflammatory cytokine and hypercatecholamine induces hematopoietic progenitor cells (HPCs) apoptosis, leading to multiorgan failure and death. However, still, result remains elusive for hematopoietic stem cells (HSCs) behavior in t...

OBJECTIVE There have been various reports on the roles of CXC receptors (CXCR) in modulation of hematopoiesis. In the present study, we investigated the effects of CXCR1 and/or CXCR2 inhibition on expansion and differentiation of umbilical cord blood (UCB) CD133(+) cells into megakaryocytic progenitors. MATERIALS AND METHODS Purified UCB CD133(+) cells were cultured in a serum-free liquid cul...

The in vitro growth of early (burst-forming unit-megakaryocyte [BFU-meg]) and late (colony-forming unit-megakaryocyte [CFU-meg]) megakaryocyte progenitors was investigated in midtrimester human fetal blood and compared with adult bone marrow. Most of the experiments were performed in a serum-free fibrin-clot assay, using purified hematopoietic progenitor (CD34+) cells. High BFU-meg and CFU-meg ...