Pancreatic beta cells exhibit oscillations in electrical activity, cytoplasmic free Ca2+ concentration ([Ca2+](i)), and insulin release upon glucose stimulation. The mechanism by which these oscillations are generated is not known. Here we demonstrate fluctuations in the activity of the ATP-dependent K+ channels (K(ATP) channels) in single beta cells subject to glucose stimulation or to stimula...

A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger beta-galactosidase in a continuous high-cell-density bioreactor. The delta-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the beta-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the delta-integration sy...

A mathematical model for collision-induced breakage is considered. Existence of weak solutions to the continuous nonlinear equation shown a large class unbounded collision kernels and daughter distribution functions, assuming kernel $K$ be given by $K(x,y)= x^{\alpha} y^{\beta} + x^{\beta} y^{\alpha}$ with $\alpha \le \beta 1$. When \in [1,2]$, it that there exists at least one mass-conserving ...

Ultrasound-guidance may help anesthesiologists to perform regional anesthesia in special situations. Bigeleisen reported an uncomplicated infraclavicular block under ultrasound guidance, in a patient having received 5000 U of heparin 2 h before the block [1]. Even if a recent report reminds us that vascular puncture remains still possible during ultrasound guided nerve blocks [2], cases have re...