Diatom algae arose by two-step endosymbiosis. The complete genome of the diatom Thalassiosira pseudonana has now been sequenced, allowing us to reconstruct the remarkable intracellular gene transfers that occurred during this convoluted cellular evolution.

Previous data suggested an active Clconductance in the renal proximal convoluted tubule, although single channel conductance and regulation were not found. We have investigated the presence and regulation of the C1channel in proximal convoluted tubules by patch clamp analysis. The current-voltage relationship of whole cells with 130 mM NaCI in the pipette was nonlinear. The addition of 1-34 PTH...

It has been established in the mammalian kidneys during the postnatal development that the primordial tubules, blastema, are piled up beneath the renal capsula, elongating themselves in order to be conjugated with the tips of the collecting tubules which had been already accomplished in embryonic life (K it t el son 1917 A r a t a k i 1926 ; Baxter and Y o f f e y 1948). The proximal convoluted...

The size, number, and location of lysosomes, phagosomes, and phago-lysosomes in different segments of the proximal and distal tubules, in the collecting tubules, and in invading macrophages of the kidneys of rats were compared by staining lysosomes (acid phosphatase) red, and phagosomes (injected horseradish peroxidase) blue in separate sections, and by staining phago-lysosomes purple by succes...

The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithel...

The distribution of vacuolar H+ATPase in rat kidney was examined by immunocytochemistry using affinity-purified antibodies against the 31-, 56-, and 70-kD subunits of the bovine kidney proton pump. Proximal convoluted tubules were labeled over apical plasma membrane invaginations, and in the initial part of the thin descending limb, apical and basolateral plasma membranes were moderately staine...

Mitochondria possess a semipermeable membrane with properties similar to the cell membrane. Despite the presence of a limiting membrane, mitochondria swell approximately 4 to 5 times their original volume without lysis or loss of internal solute. For this reason, it has been argued that the membrane might be convoluted. The present kinetic study of the permeability of isolated mitochondria was ...

INTRODUCTION The renin-angiotensin-aldosterone system (RAAS) plays an integral role in the regulation of blood pressure, electrolyte and fluid homeostasis in mammals. The capability of the different nephron segments to form components of the RAAS is only partially known. This study therefore aimed to characterize the nephron-specific expression of RAAS components within the mouse kidney. MATE...

The present study was conducted on each of the three adult birds of Emu and Duck procured from the farms located in and around Gannavaram. The renal parenchyma of Emu and Duck was divided into large cortical lobules and smaller medullary lobules/cones. The renal cortex of both the birds showed a labyrinth of tubules and renal corpuscles. Small loopless cortical (reptilian type) nephrons and lar...

This study assessed the functional role of Na(+)/H(+) exchanger (NHE) isoforms NHE3 and NHE2 in the proximal tubule, loop of Henle, and distal convoluted tubule of the rat kidney by comparing sensitivity of transport to inhibition by Hoe-694 (an agent known to inhibit NHE2 but not NHE3) and S-3226 (an agent with much higher affinity for NHE3 than NHE2). Rates of transport of fluid (J(v)) and HC...