Background: Phacoemulsification surgery is the most frequent cataract performed in developed countries because of its effectiveness and safety. However, some degree endothelial loss inevitable any type surgery, including phacoemulsification. Anterior chamber depth (ACD) could be considered as one important anatomical parameters for preserving from mechanical thermal damage that can occur during...

PURPOSE To develop standard microscopic hexagonal mosaics mimicking the human corneal endothelium for quality control of endothelial cell density (ECD) measurement and verification of cell counting strategy by light microscopy in eye banks using organ culture. METHODS A standard slide, the Keratotest, was developed with 10 laser-engraved mosaics and different predetermined "cell" densities re...

Although it is possible to observe the individual cells of the corneal endothelium with the use of the biomicroscope and specular illumination (Vogt, 1919), eye movements and the limited magnification available virtually preclude the use of this technique for systematic study of the endothelium. These problems have been largely overcome by the introduction of the specular microscope (Maurice, 1...

PURPOSE To describe corneal endothelial cell density and morphology in healthy Chinese eyes. METHODS Specular microscopy was performed in 1329 eyes of 700 healthy volunteers (M:F, 311:389), 10 to 98 years of age. Parameters studied included endothelial cell density (CD), cell area (CA), coefficient of variation (CV) in cell area, and percent hexagonality. RESULTS Mean endothelial CD in the ...

Program Number: 991 Poster Board Number: A0380 Presentation Time: 3:15 PM–5:00 PM Corneal Endothelial Cell Density in Ametropic Children and Adolescents Nicholas Faron1, James Hoekel1, Lawrence Tychsen1, 2. 1Ophthalmology, Washington Univ Sch of Med, St Louis, MO; 2Pediatrics, Washington Univ Sch of Med, St. Louis, MO. Purpose: Endothelial cell density (ECD) is known to decrease with age in adu...

Citation: Zhang W, Ogando DG, Kim ET, et al. Conditionally immortal Slc4a11 / mouse corneal endothelial cell line recapitulates disrupted glutaminolysis seen in Slc4a11 / mouse model. Invest Ophthalmol Vis Sci. 2017;58:3723–3731. DOI:10.1167/ iovs.17-21781 PURPOSE. To establish conditionally immortal mouse corneal endothelial cell lines with genetically matched Slc4a11þ/þ and Slc4a11 / mice as ...

PURPOSE To determine the viability and potential usefulness of cryopreserved human primary cultured corneal endothelial cells by characterizing their morphology, gene expression, and ability for genetic modification by the lentiviral vector equine infectious anemia virus (EIAV). METHODS Primary cultured endothelial cells were dissociated from human corneas and grown in organ culture medium. C...

Proliferative diabetic retinopathy (PDR) is a severe complication of diabetes and is a leading cause of visual decline and irreversible blindness. So we designed this study to investigate retrospectively the effect of preoperative photocoagulation on corneal endothelial cells after vitrectomy in patients with PDR.The study included 52 eyes of 46 patients with PDR complicated with vitreous hemor...

PURPOSE To evaluate short-term changes in corneal endothelial cells after trabeculectomy, EX-PRESS device implantation, and Ahmed valve implantation for the treatment of primary open-angle glaucoma. DESIGN Prospective, interventional, comparative case series with contralateral eye control study. METHODS We prospectively evaluated the changes in number, density, and shape of the corneal endo...