Primary corneal endothelial monolayers exposed to polymorphonuclear leukocytes undergo a series of morphologic alterations. Elongation occurred in foci within 3 days after removal of polymorphonuclear leukocytes, the modulated endothelial foci grew into fibroblastic colonies, and the fibroblastic cells eventually overgrew the endothelial cells. Control cultures of endothelial cells originated f...

PURPOSE To investigate cell surface-associated keratan sulfate on the corneal endothelium. METHODS Immunolabeling techniques were used at the light, scanning, and transmission electron microscopic level to localize keratan sulfate on the corneal endothelium. The investigation included human, bovine, and rabbit corneal endothelia. A quantitative study of the relationship between cell size and ...

BACKGROUND/AIMS Bacterial endotoxin is a potent inflammatory stimulator, the local and systemic responses thereby elicited being mediated via the release of cytokines from diverse cell types. Under physiological conditions, the corneal endothelium is protected from these toxins by the epithelial and vascular barriers, but in organ culture these safeguards are no longer operative, and such subst...

OBJECTIVE To determine donor risk factors for graft failure and late endothelial failure (LEF) 20 years after penetrating keratoplasty (PK). METHODS Prospective, observational study of 500 consecutive PKs performed by one surgeon. After excluding eyes undergoing second grafts, fellow eyes of bilateral cases, and patients who withdrew research authorization, 388 eyes (388 patients) were availa...

purpose: to evaluate changes in indications of corneal transplantation at labbafinejad medical center during 2001-2003. methods: records of 563 from 684 patients who had undergone corneal transplantation from oct. 2001 to oct. 2003 were evaluated. all the procedures were performed by corneal surgeons and fellows. findings: there were 292 male (51.9%) and 217 female (48.1%) subjects ranging in a...

Background: Phacoemulsification surgery is the most frequent cataract performed in developed countries because of its effectiveness and safety. However, some degree endothelial loss inevitable any type surgery, including phacoemulsification. Anterior chamber depth (ACD) could be considered as one important anatomical parameters for preserving from mechanical thermal damage that can occur during...