ATP-dependent calcium sequestration was previously localized in vesicles of mitotic apparatus isolated from sea urchins. We now demonstrate that the mitotic apparatus contains an ATP-regenerative system characterized as creatine kinase (EC 2.7.3.2). Mitotic apparatus isolated with vesicles intact converted ADP to ATP if phosphocreatine was present. Omission of ADP or phosphocreatine gave neglig...

The antigenic and physical properties of several representative invertebrate phosphagen kinases have been examined in order to further characterize the relationship between taxonomic assignment, quaternary protein structure and evolution of this class of enzymes. Antibodies against dimeric arginine kinase from the sea cucumber cross-reacted with dimeric arginine kinase purified from sea urchin ...

where ATP = adenosine triphosphate and ADP= adenosine diphosphate. The substances most commonly determined have been creatine or creatine phosphate; e.g. Banga (1943), Askonas (1951), Narayanaswami (1952), Ennor & Rosenberg (1954), Kuby, Noda & Lardy (1954) and Chappell & Perry (1954). These methods may not always be convenient for the determination of creatine phosphokinase activity in tissue ...

Serial measurements of the MB isoenzyme of creatine kinase, total creatine kinase, aspartate mainotransferase, and urea stable lactate dehydrogenase have been made in the serum of a series of 139 patients admitted with a diagnosis of suspected myocardial infarction. Serum MB isoenzyme measurements have also been made on postoperative surgical patients and patients with medical disorders known t...

Background: For patients with an increased risk of developing myopathy with statin treatment, guidelines recommend to measure CK before and 6-12 weeks after initiating statin treatment. Further, in clinical trials of statins, the number of patients experiencing elevated creatine kinase (CK) serum activity is commonly reported as a safety parameter. Usually exceeding the upper limit of normal (U...

Introduction: Male infertility is an important factor of infertility in couples. In most cases it is caused by defects in sperm cell function; whether low sperm cell count or reduced motility along with altered morphology. Defects in sperm cells development are influencing enzyme activities in the seminal fluid. Aim: This research tried to determine can activity of creatine kinase (CK) in semin...

Duchenne muscular dystrophy is an X-linked recessive disorder, affecting only males and transmitted by females, who have a 50%1 risk of an affected son or a carrier daughter. The incidence has been estimated at between one in 3000 and one in 5000 male births,' but not all the mothers of affected infants are carriers; a substantial proportion of cases will represent new mutations. According to H...

Previous studies indicate that ATP formation by the electron transport chain is impaired in sepsis. However, it is not known whether sepsis affects the mitochondrial ATP transport system. We hypothesized that sepsis inactivates the mitochondrial creatine kinase (MtCK)-high energy phosphate transport system. To examine this issue, we assessed the effects of endotoxin administration on mitochondr...

Rats were fed a 2% guanidino propionic acid diet for up to 18 weeks to induce cellular creatine depletion by inhibition of creatine uptake by this creatine analogue. Ultrastructural analysis of creatine depleted tissues showed that mitochondrial intermembrane inclusion bodies appeared in all skeletal muscles analysed, after 11 weeks of feeding. Heart had relatively few even after 18 weeks of an...