Procedures for the preparation of soluble collagen from rat skin and tail tendon were reviewed and revised to permit the preparation of native monomeric collagen with intact nonhelical ends. The degree of intactness was estimated from the tyrosine content, which is present only in the nonhelical ends, and by mobility of the COOH-terminal cyanogen bromide peptide of the alpha1 chain on sodium do...

Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilization is often used to improve biocatalyst properties, operational stability, and reusability. However, changes in the structure of biocatalysts during immobilization and under process conditions are still largely uncertain. Here, three microscopy techniques - bright-field,...

conclusions high hev seroprevalence (16.7%) was observed among the blood donors in bushehr province. it appears that exposure to hev increases with age; although, more people should be examined. results overall, 105 (16.7%) blood samples were positive for hev-specific-igg antibodies, while 523 (83.8%) were negative. the presence of anti-hev igg was not associated with gender; however, it was co...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease. the staphylococcal superantigens are considered as the causative agent of ra disease. objectives this study aimed to assess the presence of staphylococcal enterotoxin d in synovial fluid and blood of patients with ra. patients and methods a total of 120 blood and sf samples of patients with ra were studied. ba...

background the etiology of the guillain-barre syndrome (gbs) is not clear; in many cases, gbs is triggered by a bacterial or viral illness. objectives to evaluate the role of active infection with herpesviridae group and its association with this syndrome we have investigated the cerebrospinal fluid (csf) and serum of patients with gbs for detection of these viruses. patients and methods in a pr...

total igm, iga, and igm-rheumatoid factor (rf) and iga-rf were quantitated in the serum and synovial fluid of 35 seropositive and 8 seronegative rheumatoid arthritis (ra) patients, using a sensitive enzyme-linked immunosorbent assay (elisa). mononuclear cells isolated from peripheral blood and synovial fluid of three seropositive patients were also stimulated in vitro with phorbol myristate ace...

This study explores the potential application of rice bran (agro waste) to nano-encapsulate phytase, which is a thermally unstable biologically active enzyme. Rice bran was converted to nanofibers (20-50 nm in diameter) using electrospinning. After optimizing the pH, viscosity, voltage and the distance between electrodes for electrospinning, phytase enzyme was encapsulated and the fibers were c...

Enzymes are able to catalyze various specific reactions under mild conditions and can, therefore, be applied in industrial processes. To ensure process profitability, the enzymes must reusable while ensuring their enzymatic activity. improve processability immobilization of biocatalyst, can be, e.g., crystallized, resulting crystals cross-linked. These mechanically stable catalytically active p...