To understand the response of the Lyme disease spirochete Borrelia burgdorferi exposed to stress conditions and assess the viability of this spirochete, we used a correlative cryo-fluorescence and cryo-scanning microscopy approach. This approach enables simple exposition of bacteria to various experimental conditions that can be stopped at certain time intervals by cryo-immobilization, examinat...

A growing array of experimental techniques allows us to characterize the three-dimensional structure of large biological assemblies at increasingly higher resolution. In addition to X-ray crystallography and nuclear magnetic resonance in solution, new structure determination methods such cryo-electron microscopy (cryo-EM), crosslinking/mass spectrometry and solid-state NMR have emerged. Often i...

Despite great progress in the identification and characterization of the key molecular players in neuronal function, remarkably little is known about their supramolecular organization. Cryo-electron tomography (cryo-ET), providing three-dimensional views of the molecular components of the cell in their native, fully hydrated environment, is uniquely positioned to elucidate the native architectu...

Cryo-electron microscopy (cryo-EM) has produced density maps of various resolutions. Although α-helices can be detected from density maps at 5-8 Å resolutions, β-strands are challenging to detect at such density maps due to close-spacing of β-strands. The variety of shapes of β-sheets adds the complexity of β-strands detection from density maps. We propose a new approach to model traces of β-st...

Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by ...

OBJECTIVES This cross-sectional study aimed to determine the effects of exposure to cement dust on Fractional Exhaled Nitric Oxide (FeNO) in non-smoking cement mill workers. SUBJECTS AND METHODS We recruited 121 apparently healthy male volunteers, 90 of them were non-smoking cement mill workers and 31 non-smoking un-exposed subjects served as control. The mean age of cement mill workers was 3...

The purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of Rhizopus oryzae and Candida tropicalis. Olive mill waste fermentations were performed in shake and bioreactor cultures. Production of flavor compounds from olive mill waste was followed by Gas Chromatography-Mass spectrometry, Gas chromatography- olfactometry and Sp...

Market Mill is a complex dependence pattern leading to nonlinear correlations and predictability in intraday dynamics of stock prices. The present paper puts together previous efforts to build a dynamical model reflecting the market mill asymmetries. We show that certain properties of the conditional dynamics at a single time scale such as a characteristic shape of an asymmetry generating compo...

Semiautogenous (SAG) mills for ore grinding are large energy consumption equipments. The SAG energy consumption is strongly related to the fill level of the mill. Hence, on-line information of the mill fill level is a relevant state variable to monitor and drive in SAG operations. Unfortunately, due to the prevailing conditions in a SAG mill, it is difficult to measure and represent from first ...

Several months ago the speaker and Jan van Mill gave a proof of this result [W.W. Comfort, J. van Mill, Extremal pseudocompact abelian groups are compact metrizable, Abstracts Amer. Math. Soc. 27 (2006) 78 (Abstract #1014-22-958); W.W. Comfort, J. van Mill, Extremal pseudocompact abelian groups are compact metrizable, Proc. Amer. Math. Soc. 135 (2007) 4039–4044]: A pseudocompact abelian group o...