objective: evaluation of the effects of different doses of antioxidant taurine on oxidative stress and human sperm parameters following cryopreservation. materials and methods: the semen of 20 fertile men were divided to 5 aliquot, one part considered as a fresh after analysis of standard semen parameters (motility, abnormal morphology, viability) and protamine deficiency, dna damage and measu...

The scarcity of available tissue for transplantation in diabetes and the need for multiple donors make it mandatory to use an optimal cryopreservation method that allows maximal recovery and preservation of beta-cell function. We have developed a method to cryopreserve islets with excellent survival of endocrine cells. Current methods use DMSO as cryoprotectant. Our method involves introducing ...

OBJECTIVE To review the literature and answer questions about semen quality in young cancer patients before and after therapy and the importance of sperm cryopreservation. METHODS All aspects of sperm cryopreservation and effects of therapies on semen quality were examined on the basis of MedLine database searches. RESULTS Chemotherapy, radiation, or their combination results in a significa...

BACKGROUND To determine whether mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation. METHODS Immature C57BL6*BALB/c female mice underwent superovulation and the collected oocytes were divided into three groups. Group A oocytes (n = 107) underwent IVF. Group B oocytes (n = 167) underwent IVF and embryos generated were then cryopreserved. Group ...

Introduction Cryopreservation is essential for bringing tissue engineered constructs from the benchtop to the clinic. Two main methods of cryopreservation that may be used are conventional freezing and vitrification (ice-free cryopreservation). For a pancreatic substitute containing beta cells, metabolism is key to construct function (glucose-stimulated insulin secretion). However, little is kn...

OBJECTIVES Fertility preservation is not only important for malignant diseases but should also be offered to patients with autoimmune diseases (AID) like vasculitides, prior to cyclophosphamide therapy. No recommendations are available for patients with AID. METHODS Analysis from the Fertiprotekt registry of all female patients with age <40 and the diagnosis of a vasculitis. The number of cou...

While there have been several relatively recent comprehensive reviews of mammalian sperm cryopreservation (Watson, 2001; Leibo, 2004), this chapter is not meant to be a review of the literature of mammalian sperm cryopreservation. Instead, we intend to provide an understanding of the history, current status, and potential future direction of mammalian sperm cryobiology. There are many reasons w...

Adult mesenchymal stem cells (MSCs) have been investigated using preclinical approaches for tissue regeneration. Porcine MSCs (pMSCs) are capable of growing and attaching to plastic with a fibroblast-like morphology and then differentiating into bone, adipose, and cartilage tissues in vitro. This study was conducted to investigate the proliferating abilities, differentiation potentials, and mul...

PURPOSE The previous in vitro study showed that trehalose, when used as a cryoprotective agent (CPA) in an optimal concentration, can provide adequate protection of adipose aspirates during cryopreservation. OBJECTIVE The authors evaluated the efficacy of trehalose in its optimal concentration for cryopreservation of human fat grafts in a well-established animal model. METHODS In this study...