Currently there are no efficient stable transformation methods for pumpkin. In this work we report an efficient genetic transient transformation method of whole pumpkin (Cucurbita maxima cv. Big Max, a dicot) plants in the cotyledonary stage via direct inoculation of Agrobacterium tumefaciens and A. rhizogenes. A vector harboring a uidA-GFP translational fusion and the Cauliflower mosaic virus ...

In this work, the partition method introduced by Carvalho and Melo was used to study the complex between Cucurbita maxima trypsin inhibitor (CMTI-I) and glycerol at the AM1 level. An effective potential, combining non-bonding and polarization plus charge transfer (PLCT) terms, was introduced to evaluate the magnitude of the interaction between each amino acid and the ligand. In this case study,...

Natural compounds are increasingly becoming an important source of drug leads for computer-aided design approaches. Cucurbita maxima has been observed to have medicinal properties and can, therefore, be a potential novel leads. However, before can synthesized in the lab tests, modern approaches require that candidate screened drug-likeness characteristics toxicit...

This study aimed to investigate the effects of grafting on fatty acid profile and some physicochemical properties watermelon seed oil. The ‘Crimson Tide’ cultivar was used as scion while two wild (Citrullus lanatus var. citroides (A1 A2)), one Lagenaria siceraria (A3) Cucurbita maxima Duchesne x moschata (A4) were rootstocks. use rootstock significantly influenced physical parameters seeds oils...

• The stress-related phytohormones, salicylic acid (SA) and abscisic acid (ABA), and the three jasmonates, jasmonic acid (JA), cis-12-oxo-phytodienoic acid (cis-OPDA), and (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), were investigated in phloem and xylem exudates of Cucurbita maxima. • Phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry. • ...

Screening and identification of tolerant genotypes using osmotic materials under in vitro culture could be rapid, easy, even accurate. In this research, three Iranian landraces Cucurbita sp. included Tanbal Ajili (Cucurbita maxima Duch.), Razan pepo L.), Balghabakhi moschata Duch.) seeds were cultured ¼ MS medium. After germination, plantlets transferred to media containing mannitol PEG 6000. M...