The application of high-throughput sequencing of the 16S rRNA gene has increased the size of microbial diversity datasets by several orders of magnitude, providing improved access to the rare biosphere compared with cultivation-based approaches and more established cultivation-independent techniques. By contrast, cultivation-based approaches allow the retrieval of both common and uncommon bacte...

background : leishmaniasis is one of the infectious parasitic diseases of highest incidence in the world. cutaneous leishmaniasis (cl) has long been reported in shiraz, southern iran. there is a need to find a sensitive and specific method for treatment and control of the disease. methods: we have compared the sensitivity of the conventional methods microscopy and cultiva­tion of lesion scrapes...

Sponges host complex microbial communities of recognized ecological and biotechnological importance. Extensive cultivation efforts have been made to isolate sponge bacteria, but most still elude cultivation. To identify the bottlenecks of sponge bacterial cultivation, we combined high-throughput 16S rRNA gene sequencing with a variety of cultivation media and incubation conditions. We aimed to ...

decisions relating to most irrigation-planning problems need to be made in the face of hydrologic uncertainties, which make the irrigation-planning problem more complex. the uncertainties can be tackled by formulating the problem as fuzzy linear programming (flp). in the present study, single objective fuzzy linear programming (soflp) irrigation planning model was formulated for deriving the op...

Myxobacteria harbor an enormous potential for new bioactive secondary metabolites and therefore the isolation of in particular new groups is of great interest. The diversity of myxobacteria present in two ecological habitats, namely sand from Kiritimati Island and German compost, was evaluated by both cultivation-based and cultivation-independent methods. Phylogenetic analyses of the strains in...

23 The nodulation tendency and community structure of indigenous bradyrhizobia on 24 Rj-genotype soybean cultivars at cultivation temperatures of 33/28°C, 28/23°C and 23/18°C, 25 16/8 h (day/night degrees, hours) were investigated using 780 bradyrhizobial DNA samples 26 from an Andosol with 13 soybean cultivars of four Rj-genotypes (non-Rj, Rj2Rj3, Rj4 and 27 Rj2Rj3Rj4). A dendrogram was constr...

This article describes a detailed study on the background of “studying-training integration” system, a special athlete cultivation method in China. Using methods such as investigation , interview and literature review , the article searches the direction and opportunity of the reform on the cultivation system for athletes and proposes suggestions that we should restructure the sports talents cu...

This study aims to determine the current trends of paddy cultivation in Galle and Matara districts southern province Sri Lanka. Moreover, constraining factors for role extension services promote farming are also analyzed. The required data was gathered from field survey carried out using 400 farmers districts. majority lands have single ownership both while shallow bog soil dominant types field...