OBJECTIVE To evaluate the validity of western blot (WB) and enzyme linked immunosorbent assay (ELISA) that use antigens from culture promastigote from Leishmania parasites, for laboratory diagnosis of cutaneous leishmaniasis in Syria. METHODS We utilized 290 serum samples from endemic areas (patients group) and other regions (control samples) in Syria during 2002-2005 and the serological test...

There has been an increasing incidence of Lyme disease (LD) in Canada and the United States corresponding to the expanding range of the Ixodes tick vector and Lyme disease agent (Borrelia burgdorferi sensu stricto). There are many diagnostic tests for LD available in North America, all of which have some performance issues, and physicians are concerned about the appropriate use and interpretati...

The liver plays a major role in in vivo metabolisms such as in synthesis of plasma proteins, blood glucose generation, urea formation, secretion of bile, and detoxification. Since most of these hepatic functions are performed by hepatocytes, parenchymal cells of the liver, there has been much attention to their use for cell therapeutic applications and the in vitro studies of liver physiology, ...

We have compared the use of five nonvaccine antigens to the use of conventional vaccine antigens, pertussis toxin (PT), and filamentous hemagglutinin (FHA) for the serological diagnosis of pertussis by enzyme-linked immunosorbent assay (ELISA). The nonvaccine antigens included the catalytic region of adenylate cyclase toxin (CatACT), the C-terminal region of FHA (C-FHA), lipooligosaccharide (LO...

Introduction: Helicobacter pylori is a gram-negative bacillus responsible for numerous gastroduodenal pathologies, and this infection public health problem. The prevalence of with bacterium remains high in countries limited resources. Diagnosis relies mainly on invasive noninvasive methods. aim work was to evaluate the different indirect diagnostic methods using bacterial cultures. Methods: We ...

One of the most important QC tests of whole-cell pertussis vaccine (WCPV) is potency test. In this regard, mouse protection test (MPT) is the current potency method, which is associated with severe animal distress and large variability in results. The purpose of this study was to assess Pertussis Serological Potency Test (PSPT) as a serological alternative method to intracerebral challenge in M...